New NO production inhibitors from Hyssopus cuspidatus in LPS-induced RAW264.7 cells

Publication date: December 2019Source: Phytochemistry Letters, Volume 34Author(s): Dan Liu, Qiang Yin, Qihua Zhang, Jin Xiang, Changlei Ruan, Huili Liu, Bo Li, Weiliang Zhu, Chun-ping Yin, Jinbo FangAbstractTwo new sesquiterpenes, including 7-epi-3-eudesmene-1β,11-diol (1) and (7S,10R)-11-hydroxy-4,5-seco-guai-1-ene-4-one (2); one new natural product (11-hydroxy-1-oxo-4β,5β,7β,10β-eremophilane); (3) and nine known compounds were isolated from the aerial parts of Hyssopus cuspidatus Boriss. Their structures as well as their absolute configurations were elucidated by extensive spectroscopic analyses and quantum chemical calculations. Compounds 1 to 9 were tested to evaluate their inhibitory effects on NO production in LPS-induced RAW264.7 cells. Compounds 5 and 8 exhibited inhibitory effects, with IC50 values of 40.43 and 32.56 μM, respectively, compared to the positive control (i.e., dexamethasone, IC50 = 70.35 μM), and compound 4 exhibited a weaker activity, with an IC50 value of 112.56 μM.Graphical abstract
Source: Phytochemistry Letters - Category: Chemistry Source Type: research