Antibiogram, virulotyping and genetic diversity of Escherichia coli and Salmonella serovars isolated from diarrheic calves and calf handlers

Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Rasha M.A. Gharieb, Elshaima M. Fawzi, Ibrahim ElsohabyAbstractAntimicrobial resistance profile of E. coli and Salmonella serovars isolated from diarrheic calves and handlers in Egypt is unknown due to the absence of monitoring. Therefore, this study aimed to determine the virulence, genetic and antimicrobial resistance profiles of E. coli and Salmonella serovars associated with diarrhea in calves and handlers in intensive dairy farms in Egypt. A total of 36 bacterial strains (20 E. coli and 16 Salmonella) were isolated from fecal samples of 80 diarrheic Holstein dairy calves (10 E. coli and 13 Salmonella) and hand swabs of 35 handlers (10 E. coli and 3 Salmonella) in two intensive dairy farms in Sharkia Governate in Egypt. E. coli strains belonged to six different serogroups and O114:K90 was the most prevalent serogroup (30%). However, Salmonella strains were serotyped into four different serogroups and S. Kiel was the most prevalent serotype (50%). Thirteen (65%) E. coli isolates were harbouring either stx2, eaeA and/or astA virulence-associated genes. However, stn and spvC virulence genes were detected in 2 (12.5%) and 4 (25%) of Salmonella isolates, respectively. E. coli isolates showed marked resistance to ampicillin (75%), while Salmonella strains exhibited high resistance to amikacin (100%), gentamicin (93.75%) and tobramycin (87.5%). Results...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research

Related Links:

Abstract Antimicrobial resistance profile of E. coli and Salmonella serovars isolated from diarrheic calves and handlers in Egypt is unknown due to the absence of monitoring. Therefore, this study aimed to determine the virulence, genetic and antimicrobial resistance profiles of E. coli and Salmonella serovars associated with diarrhea in calves and handlers in intensive dairy farms in Egypt. A total of 36 bacterial strains (20 E. coli and 16 Salmonella) were isolated from fecal samples of 80 diarrheic Holstein dairy calves (10 E. coli and 13 Salmonella) and hand swabs of 35 handlers (10 E. coli and 3 Salmonella) i...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
syl D Abstract Turtoises are a great puzzle when it comes to their bacterial flora, the composition and structure of which are still unknown in details. Its component which has been best described so far is Salmonella spp., presumably due to the threat of reptile-associated salmonellosis in humans. This investigation tried to assess and characterize intestinal bacterial flora of imported tortoises found dead during quarantine. Most of the animals carried various serovars of Salmonella showing no antimicrobial resistance. Presence of multiresistant Escherichia coli was possibly a result of industrial breeding and h...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
Source: European Union, European Centre for Disease Prevention and Control (ECDC). Published: 5/28/2019. This 25-page report presents the results of the third round of the external quality assessment on antimicrobial susceptibility testing for national public health laboratories for Campylobacter (Campylobacter EQA3-AST) within the Food- and Waterborne Diseases and Zoonoses Network (FWD-Net). It is part of a European Union protocol for harmonized monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates. Campylobacteriosis, followed by salmonellosis, is the leading cause of zoonotic foodborne d...
Source: Disaster Lit: Resource Guide for Disaster Medicine and Public Health - Category: International Medicine & Public Health Source Type: news
Publication date: Available online 10 May 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Olga Goławska, Magdalena Zając, Aleksandra Maluta, Peter Pristas, Ľudmila Hamarová, Dariusz WasylAbstractTurtles are a great puzzle when it comes to bacterial flora, the composition and structure of which are still unknown in details. Its component which has been best described so far is Salmonella spp., presumably due to the threat of Reptile Associated Salmonellosis in humans. This investigation tried to assess and characterize intestinal bacterial flora of imported tortoises found dead d...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
Conclusion This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the viability of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion: Poultry sources are recognized as a significant cause for non-typhoidal salmonellosis. Therefore, hygienic measures should be initiated to reduce the contamination of meat and poultry products with virulent strains of Salmonella that are of public health significance. PMID: 30996829 [PubMed]
Source: Iranian Journal of Microbiology - Category: Microbiology Tags: Iran J Microbiol Source Type: research
Editorial: The Pathogenic Yersiniae–Advances in the Understanding of Physiology and Virulence, Second Edition Matthew S. Francis1* and Victoria Auerbuch2* 1Department of Molecular Biology, Umeå Centre for Microbial Research, Umeå University, Umeå, Sweden2Department of Microbiology and Environmental Toxicology, University of California, Santa Cruz, Santa Cruz, CA, United States Editorial on the Research Topic The Pathogenic Yersiniae–Advances in the Understanding of Physiology and Virulence, Second Edition Of the 18 known Yersinia species, Y. pestis, Y. pseudotuberculosis,...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In this study, a “species” is defined as a unique AMR profile, including the profile corresponding to susceptibility to all tested antimicrobials. The AMR profile (or antibiogram) of an isolate is the combination of AMR phenotype (susceptible or non-susceptible) to each drug tested. SR reflects the richness of AMR profiles without weighting of abundance. SE and SD are measures in which both SR and relative abundance are taken into account. The BP diversity index is related to the proportion of isolates with the most common AMR profile. Changes in antimicrobial testing protocol could impact upon results, partic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This report describes the dynamics of FQ-associated mutations in the highly resistant in FQ mutants in S. Enteritidis. In addition, we characterized a deletion in the ramRA integenic region, demonstrating that this frequent mutation in the highly resistant FQ mutants provide resistance or reduce susceptibility to multiple families of antibiotics. Introduction Non-typhoidal Salmonella (NTS) is a major zoonotic pathogen worldwide (Bangtrakulnonth et al., 2004; Scallan et al., 2011). Infections caused by this pathogen have been mainly associated with gastroenteritis, an acute self-limiting intestinal infection. Howeve...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
More News: Allergy & Immunology | Antimicrobial Resistance | Dairy | Egypt Health | Gastroenteritis | Genetics | Infectious Diseases | International Medicine & Public Health | Microbiology | Salmonella | Study | Veterinary Research | Zoonoses