Sensitivity of Commercially Available Influenza Rapid Diagnostic Tests in the 2018 –2019 Influenza Season

Epidemics of seasonal influenza caused by H1N1pdm09, H3N2, and type B viruses occur throughout the world. Sporadic human H5 and H7N9 virus infections are also reported in particular regions. To treat influenza patients effectively with antivirals, sensitive and broad-reactive influenza rapid diagnostic tests (IRDTs) are required. Here, we tested the sensitivity of 23 IRDTs during the 2018–2019 influenza season for their ability to detect H1N1pdm09, H3N2, H5N1, H5N6, H7N9, and Victoria- and Yamagata-lineage type B viruses. All IRDTs detected all influenza A and B viruses tested but with different sensitivities. Several IRDTs detected the H5 and H7 viruses and the seasonal viruses with similar sensitivity. Such IRDTs might be useful for diagnosing patients infected with H5 and H7 viruses.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

Related Links:

Contributors : Ariel Rodriguez-Frandsen ; Laura Martin-Sancho ; Anshu P Gounder ; Sumit K ChandaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensInfluenza A virus (IAV) is a human respiratory pathogen that causes yearly global epidemics, and sporadic pandemics due to human adaptation of pathogenic strains. Efficient replication of IAV in different species is, in part, dictated by its ability to exploit the genetic environment of the host cell. To investigate IAV tropism in human cells, we evaluated the replication of IAV strains in a diverse subset of epithelial cell lines. HeLa cells ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
Conclusions: Human challenge studies and systems biology approaches are important tools that should be used in concert to advance our understanding of influenza infection and provide targets for novel therapeutics and immunizations. Introduction Although influenza virus was recognized as an important pathogen over a century ago, influenza continues to cause a significant burden of disease. In the United States alone, it's estimated that in the 2017–2018 season there were 959,000 hospitalizations related to influenza illness, and 79,400 deaths (CDC, 2018). Worldwide, WHO estimates that annual influenza...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
The objective of this preclinical study was to assess the protective immunity induced by immunization with group 1 hemagglutinin-specific influenza virus vaccines based on chimeric hemagglutinins (cHAs) in a ferret model of influenza. The experimental designs and immunization strategies are summarized in Figure 1. Since most human adults have a primed repertoire of B cells with specificity to the HA stalk domain (19, 30), we included an influenza B virus expressing cH9/1 (B-cH9/1) to mimic pre-existing HA stalk immunity. We then compared the ability of our sequential immunization strategies to boost antibody titers against...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
AbstractPurpose of ReviewZoonotic influenza viruses are those that cross the animal-human barrier and can cause disease in humans, manifesting from minor respiratory illnesses to multiorgan dysfunction. They have also been implicated in the causation of deadly pandemics in recent history. The increasing incidence of infections caused by these viruses worldwide has necessitated focused attention to improve both diagnostic as well as treatment modalities. In this first part of a two-part review, we describe the structure of zoonotic influenza viruses, the relationship between mutation and pandemic capacity, pathogenesis of i...
Source: Current Infectious Disease Reports - Category: Infectious Diseases Source Type: research
AbstractThis work aimed to analyze the herd immunity to influenza among a Russian population living in regions with an increased risk of emergence of viruses with pandemic potential, and to isolate and investigate virus strains from severe influenza cases, including fatal cases, during the 2016 –2017 epidemic season. In November 2016 – March 2017 highly pathogenic influenza outbreaks were registered in Russia among wild birds and poultry. No cases of human infection were registered. Analysis of 760 sera from people who had contact with infected or perished birds revealed the presence o f antibodies to A(H5N1) v...
Source: Archives of Virology - Category: Virology Source Type: research
This article was originally published on The Conversation. Read the original article. type=type=RelatedArticlesblockTitle=Related... + articlesList=5919b373e4b0bd90f8e6a746,58e7ca87e4b06f8c18beeb55,58b9d3fde4b05cf0f4008d49 -- This feed and its contents are the property of The Huffington Post, and use is subject to our terms. It may be used for personal consumption, but may not be distributed on a website.
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
Conclusion This modelling study shows how the strains of influenza A – "bird flu" – circulating when a person is born give them lifelong protection against new subtypes with the same H protein groups. The researchers call this immune imprinting. This may help to explain the high severity and mortality rate seen among certain groups. For example, the massive flu pandemic of 1918 was an H1N1 strain. This had a very high fatality rate among young adults, which the researchers consider may have been because when they were born (between 1880 and 1900), H3 was the dominant strain. Therefore they had no prot...
Source: NHS News Feed - Category: Consumer Health News Tags: Heart/lungs Medical practice Source Type: news
This study indicates that quercetin showing inhibitory activity in the early stage of influenza infection provides a future therapeutic option to develop effective, safe and affordable natural products for the treatment and prophylaxis of IAV infections.
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
In this study, single-chain variable fragment (scFv) phage display technology was used to select scFv fragments recognizing influenza envelope proteins. The Tomlinson I and J scFv phage display libraries were screened against the recombinant HA2 protein (rHA2) for three rounds. Only the third-round elution sample of the Tomlinson J library showed high binding affinity to rHA2, from which three clones (3JA18, 3JA62, and 3JA78) were chosen for preparative-scale production as soluble antibody by E. coli. The clone 3JA18 was selected for further tests due to its broad affinity for influenza H1N1, H3N2 and H5N1. Simulations of ...
Source: Archives of Virology - Category: Virology Source Type: research
iou N Abstract Highly pathogenic avian influenza A H5N1 viruses remain endemic in poultry in several countries and still constitute a pandemic threat. Since the early 20th century, we experienced four influenza A pandemics. H3N2 and H1N1pdm09 viruses that respectively emerged during 1968 and 2009 pandemics are still responsible for seasonal epidemics. These viruses evolve regularly by substitutions in antigenic sites of the hemagglutinin (HA), which prevent neutralization by antibodies directed against previous strains (antigenic drift). For seasonal H3N2 viruses, an addition of N-glycosylation sites (glycosites) ...
Source: Virology - Category: Virology Authors: Tags: Virology Source Type: research
More News: Bird Flu | Epidemics | Epidemiology | H3N2 | H5N1 | Influenza | Microbiology