The Effect of Clostridium butyricum on Gut Microbiota, Immune Response and Intestinal Barrier Function During the Development of Necrotic Enteritis in Chickens

In conclusion, although C. butyricum promotes the expression of anti-inflammatory and tight junction protein genes and inhibits pro-inflammatory genes in C. perfringens-challenged chickens, it is not adequate to improve the structure of intestinal microbiota in NE chickens. Therefore, more effective schemes of C. butyricum supplementation to prevent and treat NE in chickens need to be identified.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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In this study, the expression of cspA and cspC, as well as the germination and colony formation by spores of cspAC and cspC mutants of strain SM101, were assessed. We demonstrated that 1) the cspA and cspC genes were expressed as a bicistronic operon only during sporulation in the mother cell compartment of SM101; 2) both cspAC and cspC mutant spores were unable to germinate significantly with either KCl, l-glutamine, brain heart infusion (BHI) broth, or a 1:1 chelate of Ca2+ and dipicolinic acid (DPA); 3) consistent with germination results, both cspAC and cspC mutant spores were defective in normal DPA release; 4) the co...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Publication date: Available online 9 November 2019Source: PlasmidAuthor(s): Sarah Revitt-Mills, Carmen Lao, Marie Archambault, Dena Lyras, Julian I. Rood, Vicki AdamsAbstractMany of the disease-causing toxins of the pathogenic bacterium Clostridium perfringens are harboured on large, highly stable, conjugative plasmids. Previous work has established the requirement of a ParMRC-like partitioning system for plasmid maintenance, but little is known about other mechanisms used to ensure stable plasmid inheritance. The archetypal 47 kb Tcp plasmid, pCW3, encodes a gene, resP, whose putative product has sequence similarity to ...
Source: Plasmid - Category: Biotechnology Source Type: research
In this study, the effects of spices essential oils on the growth of C. perfringens were investigated. The in vitro inhibitory activities and minimum inhibitory concentrations (MIC) of different essential oils on C. perfringens were determined using the Oxford cup and two-fold dilution method. And two models (the parameter-adjusted Gompertz kinetic model, and least square support vector machine (LS-SVM) model) were used to rapidly predict the relative growth/survival of C. perfringens in cooked chicken breast under different essential oil concentrations. The results indicated that cinnamon essential oil exhibited a notable...
Source: Food Control - Category: Food Science Source Type: research
This study was designed to determine the incidence of Clostridium perfringens and their toxin genes in children with autism spectrum disorder (ASD), and determine the antimicrobial susceptibility of C. perfringens isolates. A hundred and fourteen fecal samples were obtained from children aged 3-12 years old (57 samples from ASD children and 57 from healthy controls). Children were divided into four groups based on their gastrointestinal (GI) symptoms as follows: ASD group with and without GI symptoms, and control group with and without GI symptoms. Selective anaerobic media and VITEK®2 ANC ID card were used for isolati...
Source: Anaerobe - Category: Microbiology Authors: Tags: Anaerobe Source Type: research
Authors: Fujikawa H, Araki M PMID: 31666469 [PubMed - as supplied by publisher]
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
eol Ryu Clostridium perfringens is a Gram-positive, anaerobic, and spore forming bacterium that is widely distributed in the environment and one of the most common causes of foodborne illnesses. Bacteriophages are regarded as one of the most promising alternatives to antibiotics in controlling antibiotic-resistant pathogenic bacteria. Here we isolated a virulent C. perfringens phage, CPS1, and analysis of its whole genome and morphology revealed a small genome (19 kbps) and a short noncontractile tail, suggesting that CPS1 can be classified as a member of Picovirinae, a subfamily of Podoviridae. To determine the host r...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research
In this study, we analyzed the host responses to these toxins, including inflammasome activation, using mouse bone marrow-derived macrophages (BMDMs). Our results demonstrated, for the first time, that C. perfringens triggers the activation of caspase-1 and release of IL-1β through PFO-mediated inflammasome activation via a receptor of the Nod-like receptor (NLR) family, pyrin-domain containing 3 protein (NLRP3). The PFO-mediated inflammasome activation was not induced in the cultured myocytes. We further analyzed the functional roles of the toxins in inducing myonecrosis in a mouse model of gas gangrene. Although the...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Authors: Tolouei S, Autixier L, Taghipour M, Burnet JB, Bonsteel J, Duy SV, Sauvé S, Prévost M, Dorner S Abstract The variability of fecal microorganisms and wastewater micropollutants (WWMPs) loads in relation to influent flow rates was evaluated for a water resource recovery facility (WRRF) in support of a vulnerability assessment of a drinking water source. Incomplete treatment and bypass discharges often occur following intense precipitation events that represent conditions that deviate from normal operation. Parasites, fecal indicator bacteria, and WWMPs concentrations and flow rate were measured...
Source: Journal of Water and Health - Category: Environmental Health Tags: J Water Health Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
The objective of this study was to determine the in vitro antimicrobial activity of several organic acids and their derivatives against Gram-positive (G+) and Gram-negative (G−) bacteria. Butyric acid, valeric acid, monopropionin, monobutyrin, monovalerin, monolaurin, sodium formate, and ProPhorce—a mixture of sodium formate and formic acid (40:60 w/v)—were tested at 8 to 16 concentrations from 10 to 50,000 mg/L. The tested bacteria included G− bacteria (Escherichia coli, Salmonella enterica Typhimurium, and Campylobacter jejuni) and G+ bacteria (Enterococcus faecalis, Clostridium pe...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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