Inactivation of Listeria Monocytogenes at various growth temperatures by ultrasound pretreatment and cold plasma

Publication date: January 2020Source: LWT, Volume 118Author(s): Yuanyuan Pan, Yan Zhang, Jun-Hu Cheng, Da-Wen SunAbstractEffects of ultrasound pretreatments and dielectric barrier discharge (DBD) cold plasma on the inactivation of Listeria monocytogenes (L. monocytogenes) under various growth temperatures were investigated, and membrane fatty acid profiles of L. monocytogenes were also determined. Fatty acids anteiso-C15:0, anteiso-C17:0 and C15:0 were the most abundant fatty acids at any given growth temperature (10, 25, 37 and 42 °C). Principal components analysis (PCA) and redundancy analysis (RDA) displayed that preconditioning growth temperatures induced noticeable modifications in membrane fatty acid profile, which could be separated from each other and classified roughly into four groups. In addition, growth temperature-mediated alterations in fatty acid profile and membrane fluidity of L. monocytogenes were associated with cell viability, with a significant increase in intracellular reactive oxygen species (ROS) levels (P 
Source: LWT Food Science and Technology - Category: Food Science Source Type: research

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Publication date: Available online 19 February 2020Source: Materials Chemistry and PhysicsAuthor(s): Natasha Vieira Sievers, Liliane Damaris Pollo, Gertrudes Corção, Nilo Sérgio Medeiros CardozoAbstractThis work reports a new method to obtain polypropylene/titanium dioxide (PP/TiO2) films with antibacterial activity through the in situ synthesis of nanostructured TiO2 directly in the polymer solution. In order to compare the performance of the different types of particles, TiO2 particles were also prepared utilizing the sol-gel method and subsequently incorporated into the polymer to form PP/TiO2 films...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Publication date: Available online 19 February 2020Source: Food ControlAuthor(s): John Burnett, Sophie Tongyu Wu, Henk C. den Bakker, Peter W. Cook, Deklin R. Veenhuizen, Susan R. Hammons, Manpreet Singh, Haley F. OliverAbstractThe purpose of this study was to determine the prevalence of Listeria monocytogenes and Salmonella enterica in retail produce environments and to elucidate possible ecological niches. Thirty environmental samples per store were collected during daily operations monthly for six months in 30 retail produce departments across seven states. Selected samples were serially diluted and plated to determine ...
Source: Food Control - Category: Food Science Source Type: research
In conclusion, unlike traditional alheiras which often contain pathogenic agents, no harmful organisms nor chemical hazards were found in these new products, even though produced by the same companies.
Source: Food Control - Category: Food Science Source Type: research
Publication date: 1 June 2020Source: Chemical Engineering Journal, Volume 389Author(s): Dambarudhar Parida, Pietro Simonetti, Ruggero Frison, Ezgi Bülbül, Stefanie Altenried, Yadira Arroyo, Zoltán Balogh-Michels, Walter Caseri, Qun Ren, Rudolf Hufenus, Sabyasachi GaanAbstractA simple one-step solventless in-situ reduction method was developed to prepare silver nanoparticle (AgNP) based polymer composites using different silver precursors and thermoplastic polymers. This method utilizes the mild reducing environment of thermoplastic polymer melts to convert silver precursors to AgNPs during the extrusion pr...
Source: Chemical Engineering Journal - Category: Chemistry Source Type: research
This study aimed to evaluate the effects of modified atmospheres on the formation ability of V. parahaemolyticus and L. monocytogenes biofilms. The stress responses of bacterial biofilm formation to modified atmospheres including anaerobiosis (20% carbon dioxide, 80% nitrogen), micro-aerobiosis (20% oxygen, 80% nitrogen), and aerobiosis (60% oxygen, 40% nitrogen) were illuminated by determining the live cells, chemical composition analysis, textural parameter changes, expression of regulatory genes, etc. Results showed that the biofilm formation ability of V. parahaemolyticus was efficiently decreased, supported by the fac...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 18 February 2020Source: Microbial PathogenesisAuthor(s): Raman Karthikeyan, Pratapa Gayathri, Paramasamy Gunasekaran, Medicharla V. Jagannadham, Jeyaprakash RajendhranAbstractMembrane vesicles (MVs) are naturally secreted by many pathogenic organisms and have various functions that include the release of microbial virulence factors that contributes to pathogenesis. However, very little is known regarding the function of Gram-positive bacteria membrane vesicles. Here, we investigated the functional role of membrane vesicles of Listeria monocytogenes. We found that L. monocytogenes secreted...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
This study characterized Listeria monocytogenes, Salmonella enterica, and E. coli O157:H7 by collecting rectoanal mucosal swabs and fecal samples from 518 imported beef cattle at Jordan’s major abattoir. A unique 53 L. monocytogenes, 287 S. enterica, and 17 E. coli O157:H7 were isolated from 37, 120 and 9 different animals; respectively. The prevalence of S. enterica, L. monocytogenes and E. coli O157:H7 were 23.2% (95% CI, 19.7-27.0%), 7.1% (95% CI, 5.2-9.7%) and 1.7% (95% CI, 0.9-3.3%); respectively. All L. monocytogenes, all E. coli O157:H7 and 93.0% of S. enterica isolates resisted at least one antimicrobial clas...
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
The maturation of dendritic cells (DCs) is essential in adaptive immunity. B cell adapter for phosphoinositide 3-kinase (BCAP) has been shown a divergent activities in cell type dependent manner including B cells, NK cells, macrophages, and plasmacytoid DCs (pDCs), however, its role in conventional DCs (cDCs) remains unknown. Here, we report that BCAP negatively regulates Toll-like receptor-induced cDC maturation and inhibits cDCs from inducing antigen-specific T cell responses, thereby weakening the antibacterial adaptive immune responses of mice in a Listeria monocytogenes-infection model. Furthermore, we demonstrate tha...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
The facultative intracellular pathogen Listeria monocytogenes uses an actin-based motility process to spread within human tissues. Filamentous actin from the human cell forms a tail behind bacteria, propelling microbes through the cytoplasm. Motile bacteria remodel the host plasma membrane into protrusions that are internalized by neighboring cells. A critical unresolved...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: Biological Sciences Source Type: research
In this study, the spiced turkey breast meat (hereafter called as turkey cutlet), which is consumed at a low level in Turkey but the nutritional level of which is much closer to the red meat and which is far healthier than the meat, was cooked by means of the Sous Vide technique having many advantages. The effects of different Sous Vide cooking temperature-time combinations (65, 70, 75 °C x 20, 40, 60 min) on the physicochemical, microbiological, and sensory properties of the turkey cutlet samples were determined in detail. Based on the experimental analyzes, as the cooking temperature increased from 65 ...
Source: International Journal of Gastronomy and Food Science - Category: Food Science Source Type: research
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