Effects of antimony on enzymatic and non-enzymatic antioxidants in a metallicolous and a non-metallicolous population of Salvia spinosa L

Publication date: Available online 9 October 2019Source: Plant Physiology and BiochemistryAuthor(s): Shakiba Rajabpoor, Seyed Majid Ghaderian, Henk SchatAbstractThe present study aimed at elucidating the role of antioxidants and stress metabolites in antimony (Sb) tolerance in a metallicolous (M), Sb[V]-hypertolerant population, and a non-metallicolous (NM) population of Salvia spinosa, particularly with regard to the question of whether they could be involved in constitutive Sb tolerance or, specifically, in Sb[V] hypertolerance in the M population. Plants were exposed in hydroponics to 0, 8, 24, 74, 221 μM Sb (Ш or V). Superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) activities, and the concentrations of phenolics, flavonoids, and proline in leaves were measured after 20 d. As potential stress/tolerance markers, the concentrations of chlorophyll a and b concentration, anthocyanins, and those of total soluble and reducing sugars were also measured. Chlorophyll a concentration reflected the difference, both in Sb[III] and Sb[V] tolerance, between N and NM, and the higher toxicity of Sb[III], compared to Sb[V]. APX and proline accumulation were more induced in M than in NM, and more by Sb[V] than by Sb[III], which is theoretically compatible with a role in Sb[V] hypertolerance. CAT was more induced in M than in NM, but more by Sb[III] than Sb[V], suggesting that is not functional in Sb[V] hypertolerance. The other enzymes and compounds did not ex...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research