The dynamic emergence of GATA1 complexes identified in in vitro ES differentiation and in vivo mouse fetal liver.

The dynamic emergence of GATA1 complexes identified in in vitro ES differentiation and in vivo mouse fetal liver. Haematologica. 2019 Oct 03;: Authors: Yu X, Martella A, Kolovos P, Stevens M, Stadhouders R, Grosveld FG, Andrieu-Soler C Abstract GATA1 is an essential transcriptional regulator of myeloid hematopoietic differentiation towards red blood cells. During erythroid differentiation, GATA1 forms different complexes with other transcription factors such as LDB1, TAL1, E2A and LMO2 'he LDB1 complex') or with FOG1. The functions of GATA1 complexes have been studied extensively in definitive erythroid differentiation; however, the temporal and spatial formation of these complexes during erythroid development is unknown. We applied proximity ligation assay (PLA) to detect, localize and quantify individual interactions during ES cell differentiation and in mouse fetal liver tissue. We show that GATA1/LDB1 interactions appear before the proerythroblast stage and increases in a subset of the CD71+/TER119- cells to activate the terminal erythroid differentiation program in 12.5 day fetal liver. Using Ldb1 and Gata1 knockdown fetal liver cells, we studied the functional contribution of the GATA1/LDB1 complex during differentiation. This shows that the active LDB1 complex appears quite late at the proerythroblast stage of differentiation and confirms the power of PLA in studying the dynamic interaction of proteins in cell differentiation ...
Source: Haematologica - Category: Hematology Authors: Tags: Haematologica Source Type: research