High-Throughput Detection of Bacterial Community and Its Drug-Resistance Profiling From Local Reclaimed Wastewater Plants

This study was performed to decipher the bacterial community structure through Illumina high throughput 16S rRNA gene sequencing, and to determine the resistance profile using the Sensititre antimicrobial susceptibility test (AST) conforming to clinical lab standards (NCCLS). Out of 1747 bacterial strains detected from wastewater influent and effluent, Pseudomonas was the most predominant genus related to ESKAPE in influent, with sequence reads corresponding to 21.356%, followed by Streptococcus (6.445%), Acinetobacter (0.968%), Enterococcus (0.063%), Klebsiella (0.038%), Escherichia (0.028%) and Staphylococcus (0.004%). Despite the different treatment methods used, the effluent still revealed the presence of some Pseudomonas strains (0.066%), and a wide range of gram-positive cocci, including Staphylococcus (0.194%), Streptococcus (0.63%) and Enterococcus (0.037%), in addition to gram-negative Acinetobacter (0.736%), Klebsiella (0.1%), and Escherichia sub-species (0.811%). The AST results indicated that the strains Escherichia along with Klebsiella and Acinetobacter, isolated from the effluent, displayed resistance to 11 antibiotics, while Pseudomonas was resistant to 7 antibiotics, and Streptococcus along with Staphylococcus were resistant to 9 antibiotics. Results herein, proved the existence of some nosocomial MDR pathogens, known for ESKAPE, with potential drug resistance transfer to the non-pathogen microbes, requiring targeted remediation.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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ConclusionsST10 is considered as a high-risk clone worldwide. This type of mcr-1-harbouring clone is a major concern for human and animal health and must be under close surveillance. This study detected the presence of mcr-1 for the first time in Uruguay, albeit in an allodemic manner, associated with different antibiotic-resistance genes and from diverse clinical contexts. Considering that colistin is often the last therapeutic option available for multidrug-resistant Gram-negative bacilli infections, it is important to maximise precautions to avoid dissemination of isolates carrying mcr-1.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
This study showed that ATR-FTIR technique may be an alternative method for rapid detection of low-level vancomycin-resistantS. aureus.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research
Publication date: May 2020Source: Materials Science and Engineering: C, Volume 110Author(s): Kamal Malhotra, Sudha Shankar, Neelam Chauhan, Rajkishor Rai, Yashveer SinghAbstractSelf-assembled peptide gels have generated interest as antibacterial materials to prevent biomaterial-related infections but these peptides are often associated with poor proteolytic stability. Efforts have been made to stabilize peptides by incorporating non-natural amino acids and/or linkages but complexation with polymers have not been explored. Therefore, we developed self-assembled peptide/chitosan gels, Boc-D-Phe-γ4-L-Phe-PEA (NH007)/chi...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 16 January 2020Source: Saudi Journal of Biological SciencesAuthor(s): Suresh Dhanaraj, Somanathan Thirunavukkarasu, Henry Allen John, Sridhar Pandian, Saleh H. Salmen, Arunachalam Chinnathambi, Sulaiman Ali AlharbiAbstractThe sediment marine samples were obtained from several places along the coastline of the Tuticorin shoreline, Tamil Nadu, India were separated for the presence of bioactive compound producing actinobacteria. The actinobacterial strain was subjected to 16Sr RNA sequence cluster analysis and identified as Nocardiopsis dassonvillei- DS013 NCBI accession number: KM098151. Ba...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
In conclusion, a high prevalence of E. coli with high resistance patterns to antimicrobials was revealed in the current study, in addition to a wide distribution of their resistance determinants. These findings highlight the importance of sheep and goats as reservoirs for the dissemination of MDR E. coli and resistance gene horizontal transfer.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
In this study, the prevalence and antibiotic susceptibility test of V. parahaemolyticus in blood clams, shrimps, surf clams, and squids were determined. The overall prevalence of V. parahaemolyticus in seafood was 85.71% (120/140), consisting of 91.43% (32/35) in blood clam, 88.57% (3135) in shrimps, 82.86% (29/35) in surf clams, and 80% (28/35) in squids. The majority of V. parahaemolyticus isolates from the seafood samples were found to be susceptible to most antibiotics except ampicillin, cefazolin, and penicillin. The MAR indices of V. parahaemolyticus isolates ranged from 0.04 to 0.71 and about 90.83% of isolates were...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): L.M. Michaely, K.M. Gregor, M. Keller, M. Rissmann, R. König, B. Gutjahr, S. Dornbusch, K. Schön, C. Puff, R. Ulrich, S. Becker, M.H. Groschup, W. Baumgärtner, M. Eiden, I. Spitzbarth
Source: Journal of Comparative Pathology - Category: Pathology Source Type: research
In this study, chlorhexidine (CHX)–silver (Ag) hybrid nanoparticles (NPs) coated gauze was developed, and their bactericidal effect and in vivo wound healing capacities were tested. A new method was developed to synthesise the NPs, wherein Ag nitrate mixed with sodium (Na) metaphosphate and reduced using Na borohydride. Finally, CHX digluconate was added to form the hybrid NPs. To study the antibacterial efficacy of particles, the minimal inhibition concentration and biofilm degradation capacity against Gram-positive and Gram-negative bacteria was studied using Escherichia coli and Staphylococcus aureus. The results ...
Source: IET Nanobiotechnology - Category: Nanotechnology Source Type: research
PI3K/AKT is a vital signaling pathway in humans. Recently, several PI3K/AKT inhibitors were reported to have the ability to reverse cancer multidrug resistance (MDR); however, specific targets in the PI3K/AKT ...
Source: Molecular Cancer - Category: Cancer & Oncology Authors: Tags: Research Source Type: research
CONCLUSIONS: We observed significantly higher isolations of E-ESPAKE MDR strains in patients with hematological malignancies. PMID: 29738654 [PubMed - in process]
Source: Salud Publica de Mexico - Category: Epidemiology Authors: Tags: Salud Publica Mex Source Type: research
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