Abnormal fertility, acrosome formation, IFT20 expression and localization in the conditional Gmap210 knockout mice.

Abnormal fertility, acrosome formation, IFT20 expression and localization in the conditional Gmap210 knockout mice.
Am J Physiol Cell Physiol. 2019 Oct 02;:
Authors: Wang Z, Shi Y, Ma S, Huang Q, Yap YT, Shi L, Zhang S, Zhou T, Li W, Hu B, Zhang L, Krawetz SA, Pazour GJ, Hess RA, Zhang Z
Abstract
GMAP210 is a cis-Golgi network-associated protein and Golgi membrane receptor for IFT20, intraflagellar transport component essential for male fertility and spermiogenesis in mice. To investigate the role of GMAP210 in male fertility and spermatogenesis, floxed Gmap210 mice were bred with Stra8-iCre mice so that Gmap210 is disrupted in spermatocytes and spermatids in this study. The Gmap210flox/flox: Stra8-iCre mutant mice showed no gross abnormalities and survived to adulthood. In adult males, testis and body weights showed no difference between controls and mutant mice. Low magnification histological examination of the testes revealed normal seminiferous tubule structure but sperm counts and fertility were significantly reduced in mutant mice. Higher resolution examination of the mutant seminiferous epithelium discovered that nearly all sperm had more oblong, abnormally shaped heads, while the sperm tails appeared to have normal morphology. Electron microscopy also revealed abnormally shaped sperm heads but normal axoneme core structure; some sperm showed membrane defects in the midpiece. In mutant mice, expression levels of IFT20 and othe...

Source: Am J Physiol Cell Ph... - October 1, 2019 Category: Cytology Authors: Wang Z, Shi Y, Ma S, Huang Q, Yap YT, Shi L, Zhang S, Zhou T, Li W, Hu B, Zhang L, Krawetz SA, Pazour GJ, Hess RA, Zhang Z Tags: Am J Physiol Cell Physiol Source Type: research