Cardiomyocyte functional screening - interrogating comparative electrophysiology of high-throughput model cell systems.

Cardiomyocyte functional screening - interrogating comparative electrophysiology of high-throughput model cell systems. Am J Physiol Cell Physiol. 2019 Oct 02;: Authors: Wells SP, Waddell HM, Sim CB, Lim SY, Bernasochi GB, Pavlovic D, Kirchhof P, Porrello ER, Delbridge LMD, Bell JR Abstract Cardiac arrhythmias of both atrial and ventricular origin are an important feature of cardiovascular disease. Novel anti-arrhythmic therapies are required to overcome current drug limitations related to effectiveness and pro-arrhythmia risk in some contexts. Cardiomyocyte culture models provide a high-throughput platform forscreening anti-arrhythmic compounds, but comparative information about electrophysiological properties of commonly used types of cardiomyocyte preparations is lacking.Standardization of cultured cardiomyocyte microelectrode array (MEA) experimentation is required for its application as a high-throughput platform for anti-arrhythmic drug development. The aim of this study was to directly compare the electrophysiological properties and responses to isoproterenol of three commonly used cardiac cultures. Neonatal rat ventricular myocytes (NRVMs), immortalized atrial HL-1 cells and custom-generated human iPSC-derived cardiomyocytes (hiPSC-CMs) were cultured on MEAs for 48-120 hours. Extracellular field potentials (FPs) were recorded and conduction velocity mapped in the presence/absence of 1µM isoproterenol to assess β-adrenergic ...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research