Multivariate optimization of the refolding process of an incorrectly folded Fc-fusion protein in cell culture broth.

CONCLUSION: The refolding process increases the fraction of active protein in original broth reducing the burden of downstream purification steps markedly.
PMID: 31577202 [PubMed - as supplied by publisher]

Source: Current Pharmaceutical Biotechnology - October 1, 2019 Category: Biotechnology Authors: Behrouz H, Molavi B, Tavakoli A, Askari M, Maleknia S, Mahboudi F, Khodadadian M Tags: Curr Pharm Biotechnol Source Type: research