Super-resolution microscopy based on parallel detection

Journal of Innovative Optical Health Sciences, Ahead of Print. Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C.  J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using p ixel reassignment,” Opt. Lett. 38(15) 2889–2892 (2013)]. Here, we use a tailor-made optical fiber and 19 avalanche photodiodes (APDs) as parallel detectors to upgrade our existing confocal microscopy, termed as parallel-detection super-resolution (PDSR) microscopy. In order to obtain the correct shift value, we use the normalized 2D cross correlation to calculate the shifting value of each image. We characterized our system performance by imaging fluorescence beads and applied this system to observing the 3D structure of biological specimen.

Source: Journal of Innovative Optical Health Sciences - October 1, 2019 Category: Biomedical Science Authors: Zhimin Zhang Shaocong Liu Liang Xu Yubing Han Cuifang Kuang Yong Liu Xiang Hao Hongqin Yang Xu Liu Source Type: research