Enhancement of cisplatin-induced cytotoxicity against cervical cancer spheroid cells by targeting long non-coding RNAs.

In this study, we investigated the cytotoxic effect of cisplatin, a clinical chemotherapeutic for cervical cancer treatment, on parental and spheroid CVC cells and surveyed the effect of LncRNA on drug-resistance. We found that spheroid CVC cells showed much more resistant to cisplatin-induced cytotoxicity compared with parental CVC cells. Furthermore, cisplatin significantly induced apoptotic cell death, while it induced cell cycle arrest in G0/G1 phase at the same dose (10 μg/ml). We also found the significant expression of EGFR in spheroid instead of parental CVC cells. Interestingly, we revealed that protruding target lncRNAs were up-regulated in cisplatin-treated spheroid CVC cells, and inhibition of these lncRNAs enhanced the cytotoxicity of cisplatin against spheroid CVC cells. These data suggest that LncRNA might act as a critical modulator on drug-resistant capability of cervical CSCs and would be a novel target for cervical cancer treatment. PMID: 31570280 [PubMed - as supplied by publisher]
Source: Pathology, Research and Practice - Category: Pathology Authors: Tags: Pathol Res Pract Source Type: research