Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli Microbiology

Recent studies have shown that RNA polymerase (RNAP) is organized into distinct clusters in Escherichia coli and Bacillus subtilis cells. Spatially organized molecular components in prokaryotic systems imply compartmentalization without the use of membranes, which may offer insights into unique functions and regulations. It has been proposed that the formation...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: PNAS Plus Source Type: research

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Soft Matter, 2019, Accepted Manuscript DOI: 10.1039/C9SM01735F, PaperTapomoy Bhattacharjee, Sujit Sankar Datta Understanding how bacteria move in porous media is critical to applications in healthcare, agriculture, environmental remediation, and chemical sensing. Recent work has demonstrated that E. coli, which moves by run-and-tumble... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Soft Matter latest articles - Category: Chemistry Authors: Source Type: research
Abstract Retrospective analysis has already shown correlation between severe Chronic Periodontitis (CP) cases with human papiloma virus (HPV). Hence, we aimed to explore deep-seated infected granulation tissue removed during periodontal flap surgery procedures for residential bacterial species between HPV+ and HVP- CP cases, which may serve as good predisposition marker for oral cancer. All CP-granulation samples showed the prominence of Firmicutes, Proteobacteria, and Bacteroidetes phyla with an abundance of gram negative anaerobes, except Streptococcus. In Beta diversity nonmetric multidimensional scaling plot, ...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
In this study, the protective effects of short-chain fructo-oligosaccharides (scFOS) on oxidized oil feeding-induced intestinal dysfunction were evaluated. Piglets were fed either a control diet, an oxidized oil diet, or an oxidized oil diet supplemented with scFOS (4 g/kg diet) for 28 days. The results showed that scFOS increased the expression of tight junction proteins and decreased the production of malondialdehyde and pro-inflammatory cytokines in jejunum of oxidized oil-challenged piglets. In addition, scFOS mitigated oxidized oil-dependent changes in the relative abundances of Prevotella_9, Bacteroides, Campylob...
Source: Journal of Functional Foods - Category: Nutrition Source Type: research
In this study, we analyzed and compared the intestinal microbiota composition of patients with idiopathic focal epilepsy (n = 30) and healthy volunteer group (n = 10) by 16s ribosomal DNA sequencing. Proteobacteria phylum was found to be higher in patients with epilepsy (25.4%) than in healthy volunteers group (1.5%). The genera of Campylobacter, Delftia, Haemophilus, Lautropia, Neisseria among Proteobacteria phylum were found to be statistically significantly higher in patients with epilepsy than in healthy volunteers (p 
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: December 2019Source: Molecular Immunology, Volume 116Author(s): Tomoya Takashima, Tomoki Taku, Tomoka Yamanaka, Tamo Fukamizo, Tomoyuki Numata, Takayuki OhnumaAbstractA 38 kDa β-1,3-glucanase allergen from Cryptomeria japonica pollen (CJP38) was recombinantly produced in E. coli and purified to homogeneity with the use of Ni-affinity resin. CJP38 hydrolyzed β-1,3-glucans such as CM-curdlan and laminarioligosaccharides in an endo-splitting manner. The optimum pH and temperature for β-1,3-glucanase activity were approximately 4.5 and 50 °C, respectively. The enzyme was stabl...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 12 November 2019Source: Materials LettersAuthor(s): Li-Jun He, Jian-Chao Hao, Lin Dai, Rong-Chang Zeng, Shuo-Qi LiAbstractTo reduce the bacteria-associated infection for the Ti implant, an antibacterial coating composed of gentamicin (GS) and polyacrylic acid (PAA) was constructed using layer-by-layer (LbL) assembly technique. The surface morphology and chemical composition of the obtained multilayers were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The plate ...
Source: Materials Letters - Category: Materials Science Source Type: research
Publication date: Available online 12 November 2019Source: Enzyme and Microbial TechnologyAuthor(s): Lovaine Silva Duarte, Laísa Quadros Barsé, Pedro Ferrari Dalberto, William Tadeu Santos da Silva, Rafael Costa Rodrigues, Pablo Machado, Luiz Augusto Basso, Cristiano Valim Bizarro, Marco Antônio Záchia AyubAbstractTransglutaminases (TGases) are a class of transferases widely used in the food and biotechnology industries. In this work, we describe the production of recombinant Bacillus amyloliquefaciens TGase in Escherichia coli, obtaining the protein in its soluble and active form. In order to re...
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Conclusions Sevoflurane enhances phagocytosis of bacteria by lipopolysaccharide-challenged macrophagesin vitro andin vivo via an inducible NO synthase –dependent mechanism. Thus, sevoflurane potentiates bactericidal and antiinflammatory host-defense mechanisms in endotoxemia.
Source: Anesthesiology - Category: Anesthesiology Source Type: research
Contributors : V ânia Pobre ; Susana Barahona ; Tatiane Dobrzanski ; Maria B Steffens ; Cecília M ArraianoSeries Type : Expression profiling by high throughput sequencingOrganism : Escherichia coli K-12The transition between exponential and stationary phase is a natural phenomenon for all bacteria and requires a massive readjustment of the bacterial transcriptome. Exoribonucleases are key enzymes in the transition between the two growth phases. PNPase, RNase R and RNase II are the major degradative exoribonucleases in Escherichia coli. We analysed the whole transcriptome of exponential and stationary phases fr...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Escherichia coli K-12 Source Type: research
ConclusionESBL/AmpC-E. coli colonized the gut of travelers to Vietnam with a CTX-resistant E. coli (>107 CFU/g feces), and the colonization lasted 14 weeks.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
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