Protective antigen domain 4 of < i > Bacillus anthracis < /i > as a candidate for use as vaccine for anthrax.

Protective antigen domain 4 of <i>Bacillus anthracis</i> as a candidate for use as vaccine for anthrax. Ann Agric Environ Med. 2019 Sep 19;26(3):392-395 Authors: Żakowska D, Graniak G, Rutyna P, Naylor K, Głowacka P, Niemcewicz M Abstract Existing research for using the protective antigen (PA) of <i>Bacillus anthracis</i> as a vaccine component shows that protection against anthrax may be obtained using fragments of this protein. The aim of the research is to check whether the selected protein fragment of the protective antigen (domain 4) encoded by an appropriate nucleotide sequence of gene pag of <i>B. anthracis</i>, was expressed in the bacterial system of <i>E. coli</i>. In order to examine the selected sequence of the pag gene, a PCR reaction and a highly effective TOPO cloning strategy were used, followed by purification of the recombinant proteins and their detection by a western-blot method. In the planning of the PA4 antigen expression a higher level of effectiveness in production of small protein - domain 4 - was anticipated. As a result, the 139 amino acids protein fragment of <i>B. anthracis</i> PA (domain 4) was isolated. The research may have found the basis for in vivo research aimed at finding potential anthrax vaccine components. PMID: 31559791 [PubMed - in process]
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research