Detection of Cell Surface Ligands for Human Synovial γδ T Cells.

Detection of Cell Surface Ligands for Human Synovial γδ T Cells. J Immunol. 2019 Sep 23;: Authors: Collins C, Lui Y, Santos AM, Ballif BA, Gogerly-Moragoda AM, Brouwer H, Ross R, Balagurunathan K, Sharma S, Wright GJ, Davis S, Budd RC Abstract Lack of understanding of the nature and physiological regulation of γδ T cell ligands has considerably hampered full understanding of the function of these cells. We developed an unbiased approach to identify human γδ T cells ligands by the production of a soluble TCR-γδ (sTCR-γδ) tetramer from a synovial Vδ1 γδ T cell clone from a Lyme arthritis patient. The sTCR-γδ was used in flow cytometry to initially define the spectrum of ligand expression by both human tumor cell lines and certain human primary cells. Analysis of diverse tumor cell lines revealed high ligand expression on several of epithelial or fibroblast origin, whereas those of hematopoietic origin were largely devoid of ligand. This allowed a bioinformatics-based identification of candidate ligands using RNAseq data from each tumor line. We further observed that whereas fresh monocytes and T cells expressed low to negligible levels of TCR-γδ ligands, activation of these cells resulted in upregulation of surface ligand expression. Ligand upregulation on monocytes was partly dependent upon IL-1β. The sTCR-γδ tetramer was then used to bin...
Source: Journal of Immunology - Category: Allergy & Immunology Authors: Tags: J Immunol Source Type: research

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In 1975, researchers from Yale investigated an epidemic of 51 patients with arthritis who lived near the woodsy town of Lyme, Connecticut. The most common symptom was recurrent attacks of knee swelling. A few had pain in other joints, such as the wrist or ankle. Many had fever, fatigue, and headache. Some remembered a round skin rash before the onset of knee swelling. We now know that Lyme disease is an infection acquired from tick bites, caused by a spiral bacterium named Borrelia burgdorferi. After a tick bite, Borrelia bacteria wriggle through the skin away from the bite site. This leads to a circular red rash, known as...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Arthritis Bones and joints Infectious diseases Source Type: blogs
The objective of the study was to evaluate and compare the diagnostic accuracy (sensitivities and specificities) of serological tests that are currently in use for diagnosis of LB in clinical laboratories in Northern Europe, by use of a large serum panel. The panel consisted of 195 serum samples from well-characterized and classified patients under investigation for clinically suspected LB (n = 59) including patients with Lyme neuroborreliosis, Lyme arthritis, acrodermatitis chronica atrophicans, erythema migrans or other diseases (n = 112). A total of 201 serum samples from healthy blood donors...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
PMID: 31371661 [PubMed - in process]
Source: J Rheumatol - Category: Rheumatology Authors: Tags: J Rheumatol Source Type: research
Conditions:   Lyme Arthritis;   Lyme Disease;   Arthritis Interventions:   Drug: Naproxen;   Drug: Acetaminophen Sponsor:   Desiree Neville, MD Not yet recruiting
Source: - Category: Research Source Type: clinical trials
LYME DISEASE is known as a form of arthritis caused by bacteria that is transmitted by ticks. If detected early it can often be treated effectively. What are the three signs you may have Lyme disease?
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
This study is significant because it now allows us to systematically test the putative epitopes for their protective efficacy with an ultimate goal of selecting the most efficacious targets for development of a long-awaited LD vaccine.
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Microbial Immunity and Vaccines Source Type: research
After my tick bite I realised that the climate crisis was a more likely culprit than any conspiracy theory about weaponisationThe first indication I ’d picked up a tick-borne disease three years ago was the excruciating arthritic pain in my feet as I hobbled to a dinner party at a neighbour’s house in Jerusalem.Later that night I experienced flu-like symptoms, including a raging fever that, even with antibiotics, would last for more than a week.Continue reading...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Insects Weapons technology Wildlife Animals Science World news Environment US news Source Type: news
Lyme disease is a multisystem disorder caused by the spirochete Borrelia burgdorferi. A common late-stage complication of this disease is oligoarticular arthritis, often involving the knee. In ∼10% of cases, arthritis persists after appropriate antibiotic treatment, leading to a proliferative synovitis typical of chronic inflammatory arthritides. Here, we provide evidence...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: PNAS Plus Source Type: research
We report the identification and successful treatment of an adolescent male with Lyme arthritis who recently relocated with his family from a US Military base in Japan to Virginia. This case highlights the importance of obtaining a travel history from military members and their families who frequently relocate due to military orders. Military medical providers must be aware of tropical and travel-related illnesses and infections that may have been acquired at a prior duty station. PMID: 31247111 [PubMed - as supplied by publisher]
Source: Military Medicine - Category: International Medicine & Public Health Tags: Mil Med Source Type: research
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