Antimicrobial and antioxidative potential of free and immobilised cellobiose dehydrogenase isolated from wood degrading fungi

Publication date: Available online 24 September 2019Source: Fungal BiologyAuthor(s): Justyna Sulej, Monika Osińska-Jaroszuk, Magdalena Jaszek, Marcin Grąz, Jolanta Kutkowska, Anna Pawlik, Agata Chudzik, Renata BancerzAbstractCellobiose dehydrogenase (CDH, EC 1.1.99.18) is a glycoprotein having many biotechnological applications. In the present study, CDHs isolated from Phlebia lindtneri (PlCDH), Phanerochaete chrysosporium (PchCDH), Cerrena unicolor (CuCDH), and Pycnoporus sanguineus (PsCDH) were studied the first time for their ability to generate antioxidant and antimicrobial agents. The aim of the research was to evaluate the antioxidant and antimicrobial activity of systems composed of four CDHs and lactose or cellobiose as a reaction substrate. The free radical scavenging effect of free and immobilised enzymes was evaluated using the DPPH method. The lowest values of EC50 (10.04±0.75 μg/ml) was noted for PlCDH/lactose and for PlCDH/cellobiose (12.06±1.35 μg/ml). The EC50value reached 12.6±1.51 μg/ml in the case of PsCDH/lactose and 15.96±1.35 for PsCDH. The CDH preparations were also effectively immobilised in alginate (the immobilisation efficiency expressed as a protein yield ranged from 61.6 to 100%). The operational stability expressed as a scavenging effect showed the possibility of using the alginate beads 4 times. Both the free and immobilised CDHs as well as the CDH/substrate were tested against Gram-negative Escherichia coli ATCC 25922, Pseudomonas aeru...
Source: Fungal Biology - Category: Biology Source Type: research