Sinensetin isolated from Orthosiphon aristatus inhibits cell proliferation and induces apoptosis in hepatocellular carcinoma cells

Publication date: Available online 24 September 2019Source: Process BiochemistryAuthor(s): Divakar Samidurai, Ashok Kumar Pandurangan, Senthil KumarKrishnamoorthi, Madan Kumar Perumal, Raaman NanjianAbstractOrthosiphon aristatus is a popular traditional folk medicine extensively used in Southeast Asia for its various pharmacological effects, including antioxidant, antitumor, and hypoglycemic activities. Orthosiphon extracts have been found to be cytotoxic to hepatocellular carcinoma (HCC) cells, which is attributed to their phytochemical content. However, the mechanism of action underlying the cytotoxic effects of Sinensetin remains unclear. Hence, the present study investigated the effect of Sinensetin purified from O. aristatus on HCC in vitro. Sinensetin was isolated from O. aristatus leaves and the chemical structure was confirmed by UV-visible, IR, NMR, and ESI-MS. The results revealed that 24-h treatment with the purified compound markedly inhibited the survival of HepG2 cells, with a half maximal inhibitory concentration (IC50) of 39.93 ± 1.10 µg/mL. HepG2 cells treated with the IC50 of Sinensetin showed characteristic morphological changes, as determined by PI and AO/Etbr dual staining, including DNA fragmentation, confirmed the apoptosis induction. Sinensetin induced cell cycle arrest in the G0/G1 phase, and the data were substantiated by flow cytometry. Furthermore, Sinensetin modulated key signaling molecules; anti-apoptotic Bcl-xL expression was down-regul...
Source: Process Biochemistry - Category: Biochemistry Source Type: research