miRNA ‑205‑5p functions as a tumor suppressor by negatively regulating VEGFA and PI3K/Akt/mTOR signaling in renal carcinoma cells.

miRNA‑205‑5p functions as a tumor suppressor by negatively regulating VEGFA and PI3K/Akt/mTOR signaling in renal carcinoma cells. Oncol Rep. 2019 Sep 10;: Authors: Huang J, Wang X, Wen G, Ren Y Abstract MicroRNAs (miRNAs) are involved in the development of various types of cancers. Dysregulation of miR‑205‑5p has been reported in various types of human cancer. However, little is known concerning the role of miR‑205‑5p in renal cell carcinoma (RCC). The pr~esent study was designed to investigate the role of miR‑205‑5p in RCC. The expression of miR‑205‑5p was measured in clear cell renal cell carcinoma (ccRCC) tissues and cell lines using RT‑qPCR. RCC cell lines were transfected with miR‑205‑5p mimics. CCK‑8 assays, wound healing assays, Matrigel invasion assays and nucleosome ELISAs were used to assess the effects of miR‑205‑5p on cell growth, migration, invasion and apoptosis, respectively. Western blotting was employed to detect changes in protein levels. Bioinformatic analyses and luciferase reporter assays were performed to identify the potential targets of miR‑205‑5p. Mouse xenograft models were used to verify the effect of miR‑205‑5p in vivo. The expression of miR‑205‑5p was found to be downregulated in 25 RCC tissues compared to that noted in the adjacent normal tissues. Decreased expression of miR‑205‑5p was associated with poor clinical outcomes. Based on the results of the in...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research