Clinical significance and biological role of KLF17 as a tumour suppressor in colorectal cancer.

Clinical significance and biological role of KLF17 as a tumour suppressor in colorectal cancer. Oncol Rep. 2019 Sep 18;: Authors: Jiang X, Shen TY, Lu H, Shi C, Liu Z, Qin H, Wang F Abstract It has been reported that kruppel‑like factor 17 (KLF17) acts as a tumour suppressor in several tissues and cancer cells, however, the molecular roles, the underlying mechanisms and clinical significance of KLF17 in colorectal cancer (CRC) have not been completely elucidated. In the present study, KLF17 protein expression was detected in 140 primary CRCs and paired adjacent non‑tumour tissues using immunohistochemistry with tissue microarrays. The KLF17 mRNA expression was determined in 4 CRC cell lines and 20 pairs of the aforementioned tissues using reverse transcription quantitative polymerase chain reaction. The correlation between KLF17 expression and clinicopathologic characteristics was determined. Next, the functions of KLF17 in CRC were examined by cell proliferation, colony formation, adhesion, invasion and mouse xenograft assays. Methylation‑specific PCR and bisulfite sequencing PCR were also carried out to investigate the promoter methylation status of KLF17 in CRC cells and tissues and explore the effects of lentiviral‑mediated RNAi of UHRF1 on the methylation and expression of KLF17. The results revealed that KLF17 expression was abnormally decreased in CRC and associated with lymph node metastasis and unfavorable overall...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research