Wasf3 deficiency reveals involvement in metastasis in a mouse model of breast cancer.

Wasf3 deficiency reveals involvement in metastasis in a mouse model of breast cancer. Am J Pathol. 2019 Sep 19;: Authors: Qin H, Lu S, Thangaraju M, Cowell JK Abstract The WASF3 gene has been implicated in cancer cell movement, invasion, and metastasis by regulating genetic pathways important in these processes. Invasion and metastasis assays, however, are largely centered on xenograft models in immune compromised mice. To facilitate analysis of the role of WASF3 in spontaneous development of cancer cell metastasis, we generated a Wasf3 null strain by deleting exons 4 and 5, which encode essential motifs for Wasf3 function. On exposure to cre-recombinase a stop codon is generated immediately downstream in exon 6. Using a CMV-cre strain, Wasf3 was constitutively inactivated, which led to viable mice with no visible morphological or behavioral abnormalities. There was no abnormal development or function of the mouse mammary gland in the Wasf3 null mice and brain development was normal. In the MMTV-PyMT strain, which shows early onset breast cancer development and metastasis, Wasf3 is up-regulated in metastatic lesions. When this oncogene was introduced onto the Wasf3-null background, although metastasis was observed in these mice, there was a reduction in the number and size of metastatic lesions in the lungs. These data provide evidence for a role in WASF3 in development of metastasis in a spontaneous model of breast cancer. P...
Source: The American Journal of Pathology - Category: Pathology Authors: Tags: Am J Pathol Source Type: research