Elevated miR-615-3p expression predicts adverse clinical outcome and promotes proliferation and migration of prostate cancer cells.
In this study, we investigated the clinical and biological relevance of miR-615-3p in PC. The expression of miR-615-3p was measured in PC tissue specimens from 239 men who underwent radical prostatectomy (RP) and it was investigated if miR-615-3p could predict post-operative biochemical recurrence (BCR). These findings were subsequently validated in three independent RP cohorts (N=222, N=273, and N=387) and functional overexpression studies conducted in PC cells (PC3M). High miR-615-3p expression was significantly associated with BCR in four independent PC patient cohorts (P < 0.05, log-rank test). In addition, high miR-615-3p expression was a significant predictor of PC-specific survival (CSS) in univariate [HR 3.75, P < 0.001] and multivariate analysis after adjustment for the CAPRA-S nomogram [HR 2.66, P = 0.008] in a merged RP cohort (N=734). Moreover, overexpression of miR-615-3p in PC cells (PC3M) significantly increased cell viability, proliferation, apoptosis, and migration. Together, our results suggest that miR-615-3p is a significant predictor of post-operative BCR and CSS and has oncogenic functions in PC cells.
PMID: 31539518 [PubMed - as supplied by publisher]
Source: The American Journal of Pathology - Category: Pathology Authors: Laursen EB, Fredsøe J, Schmidt L, Strand S, Kristensen H, Ildor Rasmussen AK, Daugaard TF, Mouritzen P, Høyer S, Kristensen G, Stroomberg HV, Brasso K, Røder MA, Borre M, Sørensen KD Tags: Am J Pathol Source Type: research