Alternative method in Galleria mellonella larvae to study biofilm infection and treatment

We present a feasible method to evaluate bacterial biofilm formation in vivo that in the near future can be used to evaluate antibiofilm compounds.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research

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Publication date: 15 January 2020Source: Materials Chemistry and Physics, Volume 240Author(s): Meenakshi Sundaram Sri Abirami Saraswathi, Dipak Rana, Kumar Divya, Shanmugaraj Gowrishankar, Arunkumar Sakthivel, Subbiah Alwarappan, Alagumalai NagendranAbstractHerein, copper oxide nanoparticles (CuO NPs) are synthesized via a facile wet chemical precipitation technique and coated with poly(hexamethylenebiguanide) hydrochloride (PHMB). The surface properties of the resulting PHMB coated CuO (PHMB-c-CuO) are characterized by field emission scanning electron microscopy (FESEM) and X-ray diffraction (XRD) spectroscopy. In order t...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Publication date: Available online 5 October 2019Source: Genes &DiseasesAuthor(s): Zhiping Kuang, Guangming Dai, Ruijie Wan, Dongli Zhang, Chen Zhao, Cheng Chen, Jidong Li, Hongchen Gu, Wei HuangAbstractLev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic osteomyelitis. We have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in vivo. However, the potential mechanisms of antibacterial and osteogenic induction properties remain unclear. Thus, for osteogenesis property, immunohistochemistry, PCR, and wes...
Source: Genes and Diseases - Category: Genetics & Stem Cells Source Type: research
In this study, the silver nanoparticles (AgNPs) were synthesized using a strain Bacillus subtilis KMS2-2 by extracellularly. The effect of calcination (200 °C for 30 min) on structural characteristics and antibacterial activity of the AgNPs were investigated. The inhibitory effect of AgNPs against pathogenic bacteria such as Pseudomonas fluorescens MTCC 1749, Proteus mirabilis MTCC 425, Escherichia coli MTCC 1610, Bacillus cereus and Staphylococcus aureus MTCC 2940 was tested by agar-well diffusion method. The synthesized AgNPs before and after calcination were spherical crystalline in nature and had size range fro...
Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
Publication date: January 2020Source: LWT, Volume 117Author(s): Darla Silveira Volcan Maia, Louise Haubert, Isabela Schneid Kroning, Kauana dos Santos Soares, Thaís Larré Oliveira, Wladimir Padilha da SilvaAbstractThe aims of this study were to verify the ability of S. aureus isolates from food poisoning outbreaks to form biofilm, and evaluate the susceptibility of these isolates to Butia odorata extract (BOE) and its effect on S. aureus biofilm. All 13 S. aureus isolates formed biofilm on polystyrene; and 84.6% (11/13) carried icaA and icaB genes, 69.2% (9/13) the icaD gene, and 61.5% (8/13) the icaC gene. A...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
This study examined the inhibitory effects of zerumbone on planktonic cell growth, adhesion and biofilm formation. The results demonstrated that zerumbone remarkably inhibited mono- and dual-species biofilms formed by C. albicans and S. aureus using the XTT [2,3-bis(2-smethoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide]-reduction assay. Furthermore, a significant decrease in biomass and cell density of dual-species biofilms following zerumbone treatment was confirmed using confocal laser scanning microscopy (CLSM). Therefore, our study suggests that zerumbone is a potential antimicrobial and antibiofilm agent i...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
CONCLUSION: It has been concluded that green synthesis is an innovative technology in which natural products are used against infectious pathogens. Current research shows the significant antibacterial effect against infectious agents. PMID: 31573882 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - Category: Biotechnology Authors: Tags: Curr Pharm Biotechnol Source Type: research
Publication date: January 2020Source: Materials Science in Semiconductor Processing, Volume 105Author(s): V. Ganesan, M. Hariram, S. Vivekanandhan, S. MuthuramkumarAbstractThe Periconium sp. extract mediated sol-gel process was effectively utilized for the synthesis of ZnO nanoparticles. The aqueous fungal extract acted as chelating agent for the Zn2+ ions and lead to the formation of gel. The dried gel was characterized by X-ray diffraction analysis (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Thermogravimetric/differential thermal analysis (TG/DTA) respectively to identify th...
Source: Materials Science in Semiconductor Processing - Category: Materials Science Source Type: research
Platelets are anucleate cells produced by megakaryocytes. In recent years, a robust body of literature supports the evolving role of platelets as key sentinel and effector cells in infectious diseases, especially critical in bridging hemostatic, inflammatory, and immune continuums. Upon intravascular pathogen invasion, platelets can directly sense viral, parasitic, and bacterial infections through pattern recognition receptors and integrin receptors or pathogen: immunoglobulin complexes through Fc and complement receptors—although our understanding of these interactions remains incomplete. Constantly scanning for are...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
utarowska Studies on the functionalization of materials used for the construction of filtering facepiece respirators (FFRs) relate to endowing fibers with biocidal properties. There is also a real need for reducing moisture content accumulating in such materials during FFR use, as it would lead to decreased microorganism survival. Thus, in our study, we propose the use of superabsorbent polymers (SAPs), together with a biocidal agent (biohalloysite), as additives in the manufacturing of polypropylene/polyester (PP/PET) multifunctional filtering material (MFM). The aim of this study was to evaluate the MFM for stability...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is an emerging problem in many parts of the world. Although animal-adapted LA-MRSA has been known for many years, recent reports suggest a possible increasing trend in the zoonotic transmission of LA-MRSA in Europe. Since its emergence in the early 2000’s, several investigations have indicated that persons in prolonged, repeated contact with affected livestock are at a higher risk of becoming colonized with LA-MRSA. LA-MRSA monitoring in livestock is voluntary under current EU legislation, and not all member states, including the UK, participa...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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