IncC blaKPC-2-positive plasmid characterised from ST648 Escherichia coli
ConclusionTo our knowledge, this is the first report on complete nucleotide sequences of type 2 IncC plasmids. These findings, which hypothesise the acquisition of KPC-2-encoding transposon Tn4401a by an IncC replicon, indicate the ongoing need for molecular surveillance studies of multidrug-resistant pathogens. In addition, they underline the increasing clinical importance of the IncC plasmid family.
Authors: Bader MS, Loeb M, Leto D, Brooks AA Abstract Urinary tract infections (UTIs) caused by antibiotic- resistant Gram-negative bacteria are a growing concern due to limited treatment options. Knowledge of the common uropathogens in addition to local susceptibility patterns is essential in determining appropriate empiric antibiotic therapy of UTIs. The recommended first-line empiric antibiotic therapy for acute uncomplicated bacterial cystitis in otherwise healthy adult nonpregnant females is a 5-day course of nitrofurantion, a 3-g single dose of fosfomycin tromethamine, or a 5-day course of pevmecillinam. High...
ConclusionIt is concluded that NIT resistance in the studied NIT-RE. coli isolates was mainly mediated by nfsA and nfsB alterations.
Publication date: Available online 10 October 2019Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Bárbara Martín-Maldonado, Laura Montoro-Dasi, Maria Teresa Pérez-Gracia, Jaume Jordá, Santiago Vega, Francisco Marco-Jiménez, Clara MarinAbstractWild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial resistance in wild Bonelli’s eagles nestlings in Eastern Spain. In addition...
ConclusionGiven the widespread use of penicillins and tetracyclines in animal health, co-resistance to amoxicillin and tetracycline could be an efficient indicator of MDR in E. coli isolates. Based on a specific resistance profile and not an arbitrary number of resistances compared with MDR, this potential indicator is also precise, convenient and suitable for routine use.
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
ConclusionsOverall, this study showed that Shigella spp. are prevalent in water sources in Iran. Furthermore, the potential role of ARGs and integrons in the emergence of a MDR phenotype in Shigella isolates of water origin was demonstrated.
ConclusionThe current findings provide valuable data for understanding the multidrug resistance and pathogenic characteristics of clinical Salmonella Paratyphi A isolates.
ConclusionsClinical Enterobacteriaceae isolates, including ESBL-positive phenotypes, collected globally (excluding North America) from 2012–2016 were highly susceptible to ceftazidime/avibactam, suggesting it is a useful agent for serious infections caused by multidrug-resistant organisms belonging to the family Enterobacteriaceae when therapeutic options are limited.
Conclusion: Taken together, our data indicate that flies can be used as indicators for environmental contamination of antimicrobial resistance. More extensive studies are warranted to explore the sentinel role of flies for antimicrobial resistance.
This study report the first hypervirulent and multi-antibiotic-resistant P. multocida multilocus sequence typing strain (ST) 342 GH161213 isolated from Pekin duck in China.MethodsMIC values were confirmed by guidance of the CLSI (VET01-A4, 2013). Determination of the P. multocida GH161213 median lethal dose (LD50) by mouse models and ducklings. The plasmid pRCAD0338PM-1 was transferred to E. coli J53Azr. The whole genome sequence of P. multocida GH161213 was obtained using Illumina HiSeq 2500 system. Resistance genes were analysed using CARD databases.ResultsGH161213 is a hypervirulent strain with median lethal dose (LD50) of