Effect of Ibuprofen on Autophagy of Astrocytes During Pentylenetetrazol-Induced Epilepsy and its Significance: An Experimental Study.

This study investigated the effect and significance of ibuprofen on autophagy of astrocytes during pentylenetetrazol (PTZ) induced epilepsy. 60 male Sprague-Dawley (SD) rats were randomly divided into five groups: control group (received normal saline), PTZ group, 3-methyladenine (3-MA) + PTZ group, ibuprofen + PTZ group and 3-MA + ibuprofen + PTZ group. Dose of each agent was 35 mg/kg (PTZ), 10 mg/kg (3-MA) and 30 mg/kg (ibuprofen) and all drugs were administered intraperitoneally 15 times on alternate days (29 days). Human astrocytes were cultured in vitro. Behavioral performance (i.e., latency, grade and duration of seizures) and EEG of rats were observed and recorded. Proliferation of astrocytes was detected by CCK-8 method. Immunofluorescence and Western blot test were used to detect the expression of LC3 and GFAP. Mean number, grade and duration of seizures were markedly reduced in ibuprofen + PTZ group and 3-MA + ibuprofen + PTZ group (P 
Source: Neurochemical Research - Category: Neuroscience Authors: Tags: Neurochem Res Source Type: research

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ConclusionIt is envisaged that the current review will add value to more scientific research on M. flabellifolius and enhance/promote the increased interest in the sustainable commercialization of M. flabellifolius herbal tea as well as lead to the validation of unverified ethnobotanical claims.Graphical abstract
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
Publication date: Available online 17 January 2020Source: Neurochemistry InternationalAuthor(s): Pamela R. Westmark, Alejandra Gutierrez, Aaron K. Gholston, Taralyn M. Wilmer, Cara J. WestmarkAbstractThe ketogenic diet is highly effective at attenuating seizures in refractory epilepsy and accumulating evidence in the literature suggests that it may be beneficial in autism. To our knowledge, no one has studied the ketogenic diet in any fragile X syndrome (FXS) model. FXS is the leading known genetic cause of autism. Herein, we tested the effects of chronic ketogenic diet treatment on seizures, body weight, ketone and glucos...
Source: Neurochemistry International - Category: Neuroscience Source Type: research
Publication date: Available online 17 January 2020Source: Journal of Clinical NeuroscienceAuthor(s): Marius Gasser, Ponghatai Boonsimma, Wiracha Netbaramee, Thanin Wechapinan, Chalurmpon Srichomthomg, Chupong Ittiwut, Martin Krenn, Fritz Zimprich, Ivan Milenkovic, Angela Abicht, Saskia Biskup, Timo Roser, Vorasuk Shotelersuk, Moritz Tacke, Marianne Kuersten, Matias Wagner, Ingo Borggraefe, Kanya Suphapeetiporn, Celina von StülpnagelAbstractATP1A3 related disease is a clinically heterogeneous condition currently classified as alternating hemiplegia of childhood, rapid-onset dystonia-parkinsonism and cerebellar ataxia, ...
Source: Journal of Clinical Neuroscience - Category: Neuroscience Source Type: research
Publication date: February 2020Source: Learning and Motivation, Volume 69Author(s): Fereshteh Keikhaei, Mohammad Ali Mirshekar, Mohammad Reza Shahraki, Alireza DashipourAbstractTemporal lobe epilepsy (TLE) causes impairment in learning and memory by causing neurological damage. Using the antioxidant and anti-inflammatory effects of myricitrin (MYC), the present study was conducted in order to investigate the neuroprotection effects of MYC in kainic acid (KA) induced- TLE experimental model.Forty male Wistar rats were divided into five groups: 1: Sham, 2: MYC, 3: TLE (epileptic group), 4: VA + TLE (valproic acid and KA ...
Source: Learning and Motivation - Category: Psychiatry & Psychology Source Type: research
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Source: Cell Cycle - Category: Cytology Authors: Source Type: research
Publication date: Available online 16 January 2020Source: Epilepsy &BehaviorAuthor(s): Mary Secco
Source: Epilepsy and Behavior - Category: Neurology Source Type: research
ConclusionsClinical decisions on epilepsy or seizures in patients with such comorbidities are often handled by different specialists. A new team culture and PDTAs are needed to guarantee high standards of diagnostic and therapeutic procedures.
Source: Neurological Sciences - Category: Neurology Source Type: research
Publication date: Available online 17 January 2020Source: Neuroscience ResearchAuthor(s): Zhaofa Wu, Yulong LiAbstractPurinergic transmitters such as adenosine, ADP, ATP, UTP, and UDP-glucose play important roles in a wide range of physiological processes, including the sleep-wake cycle, learning and memory, cardiovascular function, and the immune response. Moreover, impaired purinergic signaling has been implicated in various pathological conditions such as pain, migraine, epilepsy, and drug addiction. Examining the function of purinergic transmission in both health and disease requires direct, sensitive, non-invasive too...
Source: Neuroscience Research - Category: Neuroscience Source Type: research
Publication date: Available online 16 January 2020Source: Neuroscience &Biobehavioral ReviewsAuthor(s): Willian Lazarini-Lopes, Raquel A. Do Val-da Silva, Rui M.P. da Silva-Júnior, João P. Leite, Norberto Garcia-CairascoAbstractEpilepsy is a neurological disorder characterized by the presence of seizures and neuropsychiatric comorbidities. Despite the number of antiepileptic drugs, one-third of patients did not have their seizures under control, leading to pharmacoresistance epilepsy. Cannabis sativa has been used since ancient times in Medicine for the treatment of many diseases, including convulsive sei...
Source: Neuroscience and Biobehavioral Reviews - Category: Neuroscience Source Type: research
Publication date: Available online 17 January 2020Source: Journal of Chemical NeuroanatomyAuthor(s): Hongyi LiuAbstractGrowing evidence has indicated that long noncoding RNAs (lncRNAs) are closely implicated in the progress of epilepsy. However, the expression profile and potential function of long noncoding RNAs cancer susceptibility candidate 2 (lncRNA CASC2) in epilepsy are poorly studied. The aim of this study was to testify the influence of lncRNA CASC2 on epilepsy in rat and cell models of epileptic seizure. We adopted qRT-PCR on the hippocampus of rats following pentylenetetrazol (PTZ)-stimulated epilepsy. To furthe...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research
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