Serological, molecular and hematological diagnosis in horses with clinical suspicion of equine piroplasmosis: pooling strengths
This study was carried out with samples belonging to 140 horses residing in Spain and showing common clinical signs of EP. A blood smear microscopic examination and a comparison between the different results obtained by competitive Enzyme-Linked Immunosorbent Assay (cELISA), real-time Polymerase Chain Reaction (PCR) and hematological and biochemical (direct and total bilirubin) screening were conducted. EP positivity rates by cELISA and PCR were 50.7% and 42.9%, respectively, whereas only 9% of the horses were positive in the microscopic analysis. A significantly higher number of B. caballi-positive horses were detected by cELISA than PCR, and Kappa value was higher for T. equi (k = 0.575) than for B. caballi (k = 0.401). For the first time, an association between a high ELISA inhibition percentage (IP) and a positive PCR result for B. caballi was determined. Although most authors have described T. equi as more pathogenic than B. caballi, we found that horses parasitized by B. caballi showed a more severe hemolytic anemia, whereas T. equi infections were mostly associated with leukocytosis. The hemogram and clinical chemistry could guide the veterinary surgeon towards the diagnosis of T. equi or B. caballi since horses showed a significant leukocytosis or anemia and hyperbilirubinemia, respectively; however PCR would be the test of choice in order to confirm the diagnosis. Information about the importance of a correct diagnosis of EP using a combin...
Abstract Sinogastromyzon sichangensis is a hillstream loach endemic to the upper Yangtze River, China. It is unclear whether this fish lives in a very restricted area or may be dispersed over a long distance. In the present study spatial connectivity of populations of S. sichangensis was investigated based on 343 individuals collected from 12 sites of Chishui River and employing 22 microsatellite loci. The results of genetic diversity analysis showed that observed heterozygosity (HO) and polymorphism information content (PIC) ranged from 0.5653 to 0.6999 and 0.8513 to 0.8819, respectively. Population structure ana...
Authors: Ribeiro-Filho J, Yahouédéhou SCMA, Pitanga TN, Santana SS, Adorno EV, Barbosa CG, Ferreira JRD, Pina ETG, Dos Santos Neres JS, Leite IPR, Lyra IM, Goncalves MS Abstract INTRODUCTION: Ticagrelor is an antiplatelet agent approved for the treatment of patients with an acute coronary syndrome or a history of myocardial infarction. Considering the evidence demonstrating that ticagrelor-mediated inhibition of platelet activation and aggregation have beneficial effects in the treatment of thrombotic conditions, clinical studies have been conducted to evaluate the use of this drug for the treatment o...
We examined treatment outcomes in PVT and aimed to identify predictors of incomplete recanalization and portal hypertensive complications. MATERIALS AND METHODS: This retrospective study comprised patients diagnosed with PVT between 2006 and 2015. Key exclusion criteria were liver cirrhosis, malignancy, and age
Publication date: Available online 25 September 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Maria N. Saracho Bottero, Lorenza Beati, José M. Venzal, Leonor Guardia, Carolina S. Thompson, Atilio J. Mangold, Alberto A. Guglielmone, Santiago Nava
Publication date: Available online 25 September 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Kristyna Hrazdilova, Paulina Maria Lesiczka, Jan Bardoň, Šárka Vyroubalová, Bronislav Simek, Ludek Zurek, David Modry
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Publication date: Available online 25 September 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Ning Zhang, Yixue Sun, Min Li, Yanlong Cong, Ruiyan Zhang, Jinghui Zhao
Publication date: Available online 25 September 2020Source: Ticks and Tick-borne DiseasesAuthor(s): Elisabetta Pintore, Emanuela Olivieri, Anna Maria Floriano, Davide Sassera, Nino Sanna, Giovanni Garippa
Publication date: Available online 26 September 2020Source: Physics of the Earth and Planetary InteriorsAuthor(s): N. García-Redondo, M. Calvo-Rathert, A. Carrancho, M. Bustamante-Álvarez