Serological, molecular and hematological diagnosis in horses with clinical suspicion of equine piroplasmosis: pooling strengths

This study was carried out with samples belonging to 140 horses residing in Spain and showing common clinical signs of EP. A blood smear microscopic examination and a comparison between the different results obtained by competitive Enzyme-Linked Immunosorbent Assay (cELISA), real-time Polymerase Chain Reaction (PCR) and hematological and biochemical (direct and total bilirubin) screening were conducted. EP positivity rates by cELISA and PCR were 50.7% and 42.9%, respectively, whereas only 9% of the horses were positive in the microscopic analysis. A significantly higher number of B. caballi-positive horses were detected by cELISA than PCR, and Kappa value was higher for T. equi (k = 0.575) than for B. caballi (k = 0.401). For the first time, an association between a high ELISA inhibition percentage (IP) and a positive PCR result for B. caballi was determined. Although most authors have described T. equi as more pathogenic than B. caballi, we found that horses parasitized by B. caballi showed a more severe hemolytic anemia, whereas T. equi infections were mostly associated with leukocytosis. The hemogram and clinical chemistry could guide the veterinary surgeon towards the diagnosis of T. equi or B. caballi since horses showed a significant leukocytosis or anemia and hyperbilirubinemia, respectively; however PCR would be the test of choice in order to confirm the diagnosis. Information about the importance of a correct diagnosis of EP using a combin...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research

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In this study, we detected for just the second time a SINV infection in a diseased wild bird (common wood pigeon) from Central Europe. SINV was isolated by cell culture and the complete SINV genome sequence was determined. Phylogenetic analyses revealed a close affiliation to SINV genotype I with a high similarity to human isolate sequences from Finland, Sweden and Russia. The isolate was genetically distinct from the first avian isolate suggesting the circulation of at least two different SINV strains in Germany.In order to reveal the infection frequency in SINV positive mosquito regions 749 bird blood samples were assaye...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
_______________ We are proud that Amazon India has selected The SharpBrains Guide to Brain Fitness: How to Optimize Brain Health and Performance at Any Age as part of its Kindle Weekly Deal running from October 13th to the 17th — when many bestselling eBooks will be discounted to just 49 rupees. – To benefit from the promotion, click HERE. – Readers in other countries can purchase the book at regular prices by clicking HERE. The book is also available in Spanish, Japanese, Polish and Portuguese. Book Description: “A much-needed resource to help us better understand our brains and mind...
Source: SharpBrains - Category: Neuroscience Authors: Tags: Education & Lifelong Learning Health & Wellness Amazon India brain health brain training Brain-Fitness Kindle Weekly Deal neuroplasticity Source Type: blogs
The Box-tree Moth, Cydalima perspectalis, is an Asian species of moth (usually seen in (Japan, China, Taiwan, Korea, the Russian Far East, and India), that is gradually spreading, presumably with the advent of box hedges on new housing estates, across the South East of England. Box-tree Moth, Cydalima perspectalis (Walker, 1859), to actinic light Jul 2019, VC29 It would’ve arrived as eggs/larvae on imported box (Buxus), first recorded here in 2007. Its larvae can destroy a box hedge. Another reason to go native when it comes to planting…probably too late for native box now though. I saw my first one in the tra...
Source: David Bradley Sciencebase - Songs, Snaps, Science - Category: Science Authors: Tags: Sciencebase Source Type: blogs
Journal Name: Drug Metabolism and Personalized Therapy Issue: Ahead of print
Source: Drug Metabolism and Personalized Therapy - Category: Drugs & Pharmacology Source Type: research
We report the first case of para-Bombay blood type in a Southeast Asian patient admitted at a tertiary hospital in Korea. A 23-year-old Indonesian man presented to the hospital with fever and was diagnosed with a disseminated nontuberculous mycobacterium infection and anemia. During blood group typing for blood transfusion, cell typing showed no agglutination with both anti-A and anti-B reagents. Serum typing showed strong reactivity against B cells and trace agglutination pattern with A1 cells. His red blood cells failed to react with anti-H reagents. Direct sequencing of FUT1 and FUT2 revealed a missense variation, c.328...
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
Publication date: Available online 11 October 2019Source: Thinking Skills and CreativityAuthor(s): Agurne Sampedro, Javier PeñaAbstractThe purpose of this study was to examine the role of several neurocognitive functions (executive functions, selective attention, and processing speed) as mediators in the relationship between bilingualism and creativity. The sample consisted of 224 preadolescent children, between 9 and 12 years old, residing in the Basque Country (Spain). Evaluation included neurocognitive and creativity tasks, and a linguistic proficiency questionnaire. The sample was divided into three groups depen...
Source: Thinking Skills and Creativity - Category: Science Source Type: research
ConclusionsThis predictive model of STS identifies protective factors which could be reinforced, such as harmonious passion, and risk factors which should be reduced, such as empathy and emotional effort, with a view to promoting quality of care and quality of life among these professionals.ResumenObjetivoProponer un modelo de predictores del estrés traumático secundario (ETS).DiseñoSe trata de un diseño transversal descriptivo.ÁmbitoEl estudio se llevó a cabo en las unidades de cuidados intensivos de un hospital terciario de Madrid.ParticipantesLa muestra estuvo formada por 103 pr...
Source: Medicina Intensiva - Category: Intensive Care Source Type: research
IJERPH, Vol. 16, Pages 3877: Profiles of Problematic Internet Use and Its Impact on Adolescents’ Health-Related Quality of Life International Journal of Environmental Research and Public Health doi: 10.3390/ijerph16203877 Authors: Juan M. Machimbarrena Joaquín González-Cabrera Jéssica Ortega-Barón Marta Beranuy-Fargues Aitor Álvarez-Bardón Blanca Tejero The internet has been a breakthrough for adolescents in many ways, but its use can also become dysfunctional and problematic, leading to consequences for personal well-being. The main objective is to analyze ...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Objectives: To critically assess available high-level clinical studies regarding RBC transfusion strategies, with a focus on hemoglobin transfusion thresholds in the ICU. Data Sources: Source data were obtained from a PubMed literature review. Study Selection: English language studies addressing RBC transfusions in the ICU with a focus on the most recent relevant studies. Data Extraction: Relevant studies were reviewed and the following aspects of each study were identified, abstracted, and analyzed: study design, methods, results, and implications for critical care practice. Data Synthesis: Approximately 30&...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Concise Definitive Review Source Type: research
Authors: Fousekis FS, Mitselos IV, Christodoulou DK Abstract Hepatitis E virus (HEV) is a significant health problem with approximately 20 million individuals infected annually. HEV infection has been associated with a wide spectrum of extrahepatic manifestations, including neurological, hematological and renal disorders. Guillain-Barré syndrome and neuralgic amyotrophy are the most frequent neurological manifestations. In addition, HEV infection has been observed with other neurological diseases, such as encephalitis, myelitis and Bell's palsy. Hematologic manifestations include anemia due to glucose-6-phos...
Source: Clinical and molecular hepatology - Category: Gastroenterology Tags: Clin Mol Hepatol Source Type: research
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