Validation of a previously developed quantitative polymerase chain reaction for the detection and quantification of Mycoplasma synoviae in chicken joint specimens.

Validation of a previously developed quantitative polymerase chain reaction for the detection and quantification of Mycoplasma synoviae in chicken joint specimens. Avian Pathol. 2013 Apr;42(2):100-7 Authors: Dijkman R, Feberwee A, Landman WJ Abstract A quantitative polymerase chain reaction (qPCR) was validated for the detection of Mycoplasma synoviae (PCR equivalents of colony-forming units [CFU eq.]) in chicken joint specimens with time and compared with direct mycoplasma culture. Joint specimens were obtained from 70 layer pullets inoculated intravenously with M. synoviae at 6 weeks of age. Ten control birds were injected intra-articularly with Freund's complete adjuvant. Macroscopic joint lesions were observed in 54 infected birds, of which 11 showed positive M. synoviae culture. The specificity of direct mycoplasma culture was high (100%, 95% confidence interval [CI] = 74 to 100), but its sensitivity low (16%, 95% CI = 8 to 26). Most positive results were obtained during the first 2 weeks after onset of joint swelling using synovial fluid. The qPCR was positive in 26 of 28 synovial fluid samples and in 51 of 70 joint swabs. The sterile joint samples obtained from Freund's complete adjuvant-injected birds were negative in the mycoplasma culture. The specificity and sensitivity of the qPCR for synovial fluid samples were 100% (95% CI = 65 to 100) and 93% (95% CI = 77 to 99); for joint swabs they were 100% (95% CI = 74 to 100) and 73% (95% CI = 61 to 83), r...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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In this study, we detected for just the second time a SINV infection in a diseased wild bird (common wood pigeon) from Central Europe. SINV was isolated by cell culture and the complete SINV genome sequence was determined. Phylogenetic analyses revealed a close affiliation to SINV genotype I with a high similarity to human isolate sequences from Finland, Sweden and Russia. The isolate was genetically distinct from the first avian isolate suggesting the circulation of at least two different SINV strains in Germany.In order to reveal the infection frequency in SINV positive mosquito regions 749 bird blood samples were assaye...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, we detected for just the second time a SINV infection in a diseased wild bird (common wood pigeon) from Central Europe. SINV was isolated by cell culture and the complete SINV genome sequence was determined. Phylogenetic analyses revealed a close affiliation to SINV genotype I with a high similarity to human isolate sequences from Finland, Sweden and Russia. The isolate was genetically distinct from the first avian isolate suggesting the circulation of at least two different SINV strains in Germany.In order to reveal the infection frequency in SINV positive mosquito regions 749 bird blood samples were assaye...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
AbstractAvian reovirus (ARV) is a member of the genusOrthoreovirus in the familyReoviridae and causes a severe syndrome including viral arthritis that leads to considerable losses in the poultry industry. Innate immunity plays a significant role in host defense against ARV. Here, we explored the interaction between ARV and the host innate immune system by measuring mRNA expression levels of several genes associated with the MDA5 signaling pathway. The results showed that expression peaks for MDA5, MAVS, TRAF3, TRAF6, IRF7, IKK ɛ, TBK1 and NF-κB occurred at 3 days postinfection (dpi). Moreover, type I IFN (IFN-&alpha...
Source: Archives of Virology - Category: Virology Source Type: research
We examined 9293 individuals from the Copenhagen General Population Study using nuclear magnetic resonance spectroscopy measurements of total cholesterol, free- and esterified cholesterol, triglycerides, phospholipids, and particle concentration. Fourteen subclasses of decreasing size and their lipid constituents were analysed: six subclasses were very low-density lipoprotein (VLDL), one intermediate-density lipoprotein (IDL), three low-density lipoprotein (LDL), and four subclasses were high-density lipoprotein (HDL). Remnant lipoproteins were VLDL and IDL combined. Mean nonfasting cholesterol concentration was 72...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In this study, ARVs from the tendon of 23 broiler flocks with marked tenosynovitis were characterized, and their genetic relationship was examined. Analysis of the amino acid sequence of Sigma C protein revealed that all ARVs detected in affected broiler flocks share genetic homogeneity and suggests that a single genotype is involved in recent outbreaks. This genotype, so-called "Ardehal strain," is grouped in cluster I with vaccine strains. The amino acid sequence similarity between Ardehal and vaccine strain, including S1133, 1733, and 2408 was less than 80%. As the outbreaks have occurred in progenies of vacci...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
Qiancheng Deng1, Yangyang Luo1,2, Christopher Chang3, Haijing Wu1, Yan Ding4* and Rong Xiao1* 1Hunan Key Laboratory of Medical Epigenetics, Department of Dermatology, The Second Xiangya Hospital, Central South University, Changsha, China 2Department of Dermatology, Hunan Children's Hospital, Changsha, China 3Division of Rheumatology, Allergy and Clinical Immunology, University of California, Davis, Davis, CA, United States 4Department of Dermatology, Hainan Provincial Dermatology Disease Hospital, Haikou, China Autoimmune diseases are usually complex and multifactorial, characterized by aberrant produc...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Jennifer M. Rudd1, Sivasami Pulavendran1, Harshini K. Ashar1, Jerry W. Ritchey1, Timothy A. Snider1, Jerry R. Malayer1, Montelongo Marie1, Vincent T. K. Chow2 and Teluguakula Narasaraju1* 1Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK, United States2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore, Singapore Exaggerated host innate immune responses have been implicated in severe influenza pneumonia. We have previously demonstrated that excessive neutrophils recruited during in...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Molecular characterization of emerging avian reovirus variants isolated from viral arthritis cases in Western Canada 2012-2017 based on partial sigma (σ)C gene. Virology. 2018 Jul 17;522:138-146 Authors: Palomino-Tapia V, Mitevski D, Inglis T, van der Meer F, Abdul-Careem MF Abstract Viral Arthritis (VA), a disease caused by Avian Reovirus (ARV), has emerged as a significant cause of economic losses in broiler chicken flocks in Western Canada. These outbreaks were characterized by 4-13% morbidity, followed by a spike in mortality/culling that in extreme cases required total flock depopulation. F...
Source: Virology - Category: Virology Authors: Tags: Virology Source Type: research
In vitro study on role of σB protein in avian reovirus pathogenesis. Oncotarget. 2018 Apr 13;9(28):19569-19583 Authors: Praharaj MR, Sahoo AP, Chauhan TKS, Gandham RK, Saxena S, Agarwal RK, Dhama K, Mishra B, Marriappan AK, Tiwari AK, Goswami PP, Mishra BP, Kumar D Abstract Avian reoviruses, members of Orthoreovirus genus was known to cause diseases like tenosynovitis, runting-stunting syndrome in chickens. Among eight structural proteins, the proteins of S-class are mainly associated with viral arthritis but the significance of σB protein in arthritis is not established till date. In this ...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reovirus strain S1133 and reoviruses of Hungarian, Chinese, and US origin had occurred. In addition, recombination analyses showed evidence of intra-segmental recombination in theM2 andS2 genes. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutat...
Source: Archives of Virology - Category: Virology Source Type: research
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