In vivo screening of five phytochemicals/extracts and a fungal immunomodulatory protein against colibacillosis in broilers.

In conclusion, none of the tested phytochemicals/extracts and the FIP significantly reduced the E. coli-induced mortality and generalized colibacillosis, and nor did they improve recovery from colibacillosis lesions. PMID: 23627727 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research

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Publication date: Available online 31 October 2019Source: Veterinary MicrobiologyAuthor(s): Xiangkai Zhuge, Min Jiang, Fang Tang, Yu Sun, Yiming Ji, Feng Xue, Jianluan Ren, Weiyun Zhu, Jianjun DaiAbstractColisepticemia caused by bloodstream infection of the extraintestinal pathogenic Escherichia coli (ExPEC) has become a serious public health problem. The recent emergence of the colistin-resistant Enterobacteriaceae, especially mcr-1-positive E. coli (MCRPEC) exerts great concern around the world. The molecular epidemiology and zoonosis risk of avian-origin MCRPEC are reported to be substantially lower. Here, we presented ...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
In this study, we examined the bactericidal activity of chicken serum and blood, as well as bacterial survival in HD11 macrophages. We compared the transcriptome of ybjX mutations with those of the wild strain and studied the effects of ybjX on miRNA expression in the spleen. Our findings revealed that the mutant strain, ΔybjX, had a lower resistance to chicken serum and blood, as well as bacterial survival in HD11 macrophages than AE17. RNA sequencing analyses showed that the ybjX mutation reduced stress resistance by down-regulating mRNAs in metabolic pathways. Infection with the ybjX mutation caused changes in the...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 108 bacteria directly into the oviduct using a surgical procedure. After an infection peri...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
To better understand public health implications of waterfowl as reservoirs for zoonotic sources of Campylobacter in recreational waters, we developed a Gallus gallus (chick) model of infection to assess the pathogenicity of environmental isolates of Campylobacter. This method involved exposure of 1-day-old chicks through ingestion of water, the natural route of infection. Viable Campylobacter from laboratory-infected animals were monitored by using a modified non-invasive sampling of fresh chick excreta followed by a passive polycarbonate-filter migration culture assay. The method was used to evaluate the infectivities of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Campylobacter jejuni (C. jejuni) are able to colonise and infect domestic poultry and also pose a risk for humans. The aim of this study was to determine the extent of genotypic diversity among C. jejuni isolates recovered from avian and human sources in Egypt. Furthermore, the short variable region (SVR) of flagellin A (flaA) gene was analysed for the presence of allelic variants. Our results showed that C. jejuni isolates differ in their capacity to harbour each of the virulence genes alone or when present in various combinations. The flaA gene was detected in all C. jejuni strains and none of the strains had all the stu...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The objective of the current study was to compare the ability of the well-characterized UPEC strain, UTI89, and the APEC strain, F149H1S2, to infect human and avian cells in culture and to cause salpingitis in an infection model in adult laying hens. In vitro characterization showed that the strains grew equally well in human urine, and both were able to infect human intestinal (Int407) and bladder (J82) epithelial cell lines, and they survived in avian macrophages (HD11) to the same extent. Groups of adult birds were inoculated with 108 bacteria directly into the oviduct using a surgical procedure. After an infection peri...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Abstract Campylobacter jejuni and C. coli are leading causes of human foodborne illness with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to examine survival of Campylobacter, including different strains, in freshly-excreted feces from naturally-colonized commercial turkey flocks and in suspensions of turkey feces in water from the turkey house. Fecal and water sus...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
In conclusion, mcr-1-positive E. coli environmental isolates were mostly characterised by positive for APEC-associated virulence genes. Co-presence of those genes may suggest the existence of common source in animals and/or their associated environments.IMPORTANCE Colistin is considered as a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing Enterobacteriaceae and multidrug-resistant Acinetobacter baumannii Increasing prevalence of mcr genes in diverse Enterobacteriaceae species, mainly E. coli and Klebsiella pneumoniae from humans and ...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
The highly pathogenic (HP) avian influenza virus (AIV), H5N1 and reassortant H5-subtype HPAIVs, H5N2, H5N6, and H5N8, cause high mortality in domestic birds, resulting in economic losses in the poultry industry. H5N1 and H5N6 also pose significant public health risks and H5N1 viruses are a permanent pandemic threat. To control HPAIVs, eukaryotic expression systems have traditionally been exploited to produce vaccines based on hemagglutinin (HA), a protective viral antigen. In contrast, we used a bacterial expression system to produce vaccine targeting the HA protein. A fragment of the HA ectodomain from H5N1, with a multib...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
In this study, we found that the GroEL protein (heat shock protein 60) of Mycoplasma gallisepticum could induce apoptosis in peripheral blood mononuclear cells, and the underlying molecular mechanism was further determined. The GroEL gene from Mycoplasma gallisepticum was cloned and expressed in Escherichia coli to facilitate the functional analysis of recombinant protein. The purified GroEL protein was shown to adhere to peripheral blood mononuclear cells (PBMCs) and DF-1 cells and cause apoptosis in PBMCs. A protein pulldown assay coupled with mass spectrometry identified that annexin A2 possibly interacted with GroEL pr...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
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