Collagen type X is essential for successful mesenchymal stem cell-mediated cartilage formation and subsequent endochondral ossification.

Collagen type X is essential for successful mesenchymal stem cell-mediated cartilage formation and subsequent endochondral ossification. Eur Cell Mater. 2019 Sep 18;38:106-122 Authors: Knuth CA, Andres Sastre E, Fahy NB, Witte-Bouma J, Ridwan Y, Strabbing EM, Koudstaal MJ, van de Peppel J, Wolvius EB, Narcisi R, Farrell E Abstract n tissue engineering, endochondral ossification (EO) is often replicated by chondrogenically differentiating mesenchymal stromal cells (MSCs) in vitro and achieving bone formation through in vivo implantation. The resulting marrow-containing bone constructs are promising as a treatment for bone defects. However, limited bone formation capacity has prevented them from reaching their full potential. This is further complicated since it is not fully understood how this bone formation is achieved. Acellular grafts derived from chondrogenically differentiated MSCs can initiate bone formation; however, which component within these decellularised matrices contribute to bone formation has yet to be determined. Collagen type X (COLX), a hypertrophy-associated collagen found within these constructs, is involved in matrix organisation, calcium binding and matrix vesicle compartmentalisation. However, the importance of COLX during tissue-engineered chondrogenesis and subsequent bone formation is unknown. The present study investigated the importance of COLX by shRNA-mediated gene silencing in primary MSCs. A significant knock-down of COLX d...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research

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Source: Stem Cell Research - Category: Stem Cells Source Type: research
Authors: Freeman FE, Browe DC, Nulty J, Von Euw S, Grayson WL, Kelly DJ Abstract Interconnected porosity is critical to the design of regenerative scaffolds, as it permits cell migration, vascularisation and diffusion of nutrients and regulatory molecules inside the scaffold. 3D printing is a promising strategy to achieve this as it allows the control over scaffold pore size, porosity and interconnectivity. Thus, the aim of the present study was to integrate distinct biofabrication strategies to develop a multiscale porous scaffold that was not only mechanically functional at the time of implantation, but also faci...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research
Contributors : Michael V Frochaux ; Maroun B Sleiman ; Bart DeplanckeSeries Type : Expression profiling by high throughput sequencingOrganism : Drosophila melanogasterResistance to enteric pathogens is a complex trait at the crossroads of multiple biological processes. We have previously shown in the Drosophila Genetic Reference Panel (DGRP) that resistance to infection is highly heritable, but our understanding of how the effects of genetic variants are channeled through distinct molecular layers is still limited. To address this, we employed a systems genetics approach on the gut transcriptomes from 38 control and orally...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Drosophila melanogaster Source Type: research
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Source: Life Sciences - Category: Biology Source Type: research
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Source: Arthroscopy Techniques - Category: Surgery Source Type: research
This study demonstrated that the application of lovastatin enhanced the osteogenic differentiation of cell spheroids formed from GDSCs. This suggests that combinations of lovastatin and stem cell spheroids may have the potential for use in tissue engineering. PMID: 31602217 [PubMed]
Source: Experimental and Therapeutic Medicine - Category: General Medicine Tags: Exp Ther Med Source Type: research
Conclusions: PICU mortality of pediatric cancer and hematopoietic stem cell transplant patients requiring continuous renal replacement therapy is sadly high. Fluid overload at the initiation of continuous renal replacement therapy is the most important and earliest predictor of PICU mortality. Our results suggest that the most commonly used criteria of acute kidney injury, that is, serum creatinine and urine production, are not useful as a trigger to initiate continuous renal replacement therapy. This highlights the urgent need for prospective studies to generate recommendations for effective therapeutic interventions at...
Source: Critical Care Medicine - Category: Emergency Medicine Tags: Online Clinical Investigations Source Type: research
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Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
Publication date: Available online 11 October 2019Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Jennifer C. Zhao, Justin R. Arnall, Allison L. Martin, Saad Usmani, Shebli Atrash
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
Publication date: Available online 11 October 2019Source: Hematology/Oncology and Stem Cell TherapyAuthor(s): Reyad Dada
Source: Hematology Oncology and Stem Cell Therapy - Category: Cancer & Oncology Source Type: research
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