Hot Water Sanitization of a Commercial Mushroom Disk Slicer to Inactivate Listeria monocytogenes

In this study, the efficacy of hot water disinfection treatments to eliminate L. monocytogenes from commercial mushroom slicers was examined. Fourteen L. monocytogenes strains, including several obtained from mushrooms or mushroom processing environments, were screened for heat tolerance at 60 oC for 6 min. The 6 most heat tolerant strains were used to prepare a microbial cocktail for thermal inactivation studies. Heat tolerance of planktonic cells was not affected by incubation at 30 °C for up to 7 days, or the extent to which they adhered to stainless-steel coupons under the same conditions. Respective L. monocytogenes D50, D60, and D70-values of 11.5 min, 1.90 min, and 1.0 min were not higher than D-values for a L. innocua isolate at the same temperatures. Heat penetration studies, conducted on the slicer head immersed in a heated clean-out-of-place (COP) wash tank, determined that the slowest-to-heat food contact location (cold spot) was the at the interface of one of the blade-spacers with the horizontal slicer drive shaft. A microbial challenge study was conducted by disassembling the slicer head, inoculating the cold spot with 7 log cells L. innocua surrogate, and subjecting the reassembled slicer head to water temperatures of 55 oC, 65 oC, or 75 oC for 93, 16.4, or 6.5 min. Complete elimination of L. innocua cells for each slicer head treatment demonstrated the feasibility of hot water sanitization treatments to minimize L. monocytogenes food safety risks.
Source: Food Control - Category: Food Science Source Type: research

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Source: LWT Food Science and Technology - Category: Food Science Source Type: research
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Source: Food Control - Category: Food Science Source Type: research
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Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
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Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
Scobie et al. recently reported a 10-year review of non-pregnant listeriosis cases in England.1 No case of cutaneous listeriosis was reported in this large cohort of 1,357 patients. Aside gastro-enteritis and invasive infections (septicemia, neurolisteriosis and maternal-neonatal infections), Listeria monocytogenes (Lm) can also be responsible for other focal infections, including bone and joint infection, biliary, pulmonary, or urinary tract infections.2 Skin infections have also been reported before, either as a part of disseminated infection in neonates —a condition known as “granulomatosis infantiseptica&rd...
Source: Journal of Infection - Category: Infectious Diseases Authors: Tags: Letter to the Editor Source Type: research
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Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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