Synthesis and characterization of 4'-C-guanidinomethyl-2'-O-methyl-modified RNA oligomers.

This study investigated the synthesis and properties of 4'-C-guanidinomethyl-2'-O-methyluridine and RNAs containing the analog. Thermal and thermodynamic stabilities of double-stranded RNAs (dsRNAs) containing the nucleoside analog were examined. It was found that although the analog decreased the thermal and thermodynamic stabilities of dsRNA, it had base-discrimination ability. The 4'-C-guanidinomethyl modification increased stability of RNAs in a buffer containing serum. Furthermore, small interference RNAs incorporating one analog at the passenger strand still preserved their RNA interference activities. It was suggested that the 4'-guanidinomethyl modification significantly improved cell membrane permeability of RNA. Thus, 4'-C-guanidinomethyl-2'-O-methyl analogs may be useful in improving the properties of therapeutic siRNA molecules.
PMID: 31530080 [PubMed - as supplied by publisher]

Source: Nucleosides, Nucleotides and Nucleic Acids - September 20, 2019 Category: Biochemistry Tags: Nucleosides Nucleotides Nucleic Acids Source Type: research