Association between promoter DNA methylation and gene expression in the pathogenesis of ischemic stroke.

Association between promoter DNA methylation and gene expression in the pathogenesis of ischemic stroke. Aging (Albany NY). 2019 Sep 17;11: Authors: Deng GX, Xu N, Huang Q, Tan JY, Zhang Z, Li XF, Wei JR Abstract To assess DNA methylation sites as well as gene expression related to ischemic stroke (IS) and comprehensively reveal their correlation and possible pathological mechanisms, we implemented (1) genome-wide DNA methylation profiling from the GEO repository related to IS with and without symptoms; (2) identification of differentially methylation positions (DMPs) and genes (DMGs), functional enrichment analysis along with DMG regulatory network construction; (3) validation tests of 2 differential methylation positions of interest as well as analogous gene expression in other datasets and in IS patients and controls; and (4) correlation analysis of DNA methylation and mRNA expression data. In total, 870 DMPs were physically located within 693 DMGs. After disease ontology (DO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, gene ontology (GO), protein-protein interaction (PPI) network construction as well as module analysis, HLA-DRB1 and HLA-DQB1 were identified. Their expression was validated in 4 other datasets but was significant in only 1, and the expression was lower in the IS group (P < 0.05). After validation in IS patients and controls, we found that these two genes showed more hypermethylation and lower expres...
Source: Aging - Category: Biomedical Science Authors: Tags: Aging (Albany NY) Source Type: research