Wasf3 deficiency reveals involvement in metastasis in a mouse model of breast cancer

The WASF3 gene has been implicated in cancer cell movement, invasion, and metastasis by regulating genetic pathways important in these processes. Invasion and metastasis assays, however, are largely centered on xenograft models in immune compromised mice. To facilitate analysis of the role of WASF3 in spontaneous development of cancer cell metastasis, we generated a Wasf3 null strain by deleting exons 4 and 5, which encode essential motifs for Wasf3 function. On exposure to cre-recombinase a stop codon is generated immediately downstream in exon 6.
Source: American Journal of Pathology - Category: Pathology Authors: Source Type: research