Interaction of ArmZ with the DNA-binding domain of MexZ induces expression of mexXY multidrug efflux pump genes and antimicrobial resistance in Pseudomonas aeruginosa.

Interaction of ArmZ with the DNA-binding domain of MexZ induces expression of mexXY multidrug efflux pump genes and antimicrobial resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2019 Sep 16;: Authors: Kawalek A, Modrzejewska M, Zieniuk B, Bartosik AA, Jagura-Burdzy G Abstract Multidrug efflux pumps play an important role in antibiotic resistance in bacteria. In Pseudomonas aeruginosa, MexXY pump provides intrinsic resistance to many antimicrobials, including aminoglycosides. The expression of mexXY operon is negatively regulated by MexZ repressor. The repression is alleviated in response to the antibiotic-induced ribosome stress, which results in increased synthesis of anti-repressor ArmZ, interacting with MexZ. The molecular mechanism of MexZ inactivation by ArmZ is not known. Here, we showed that the N-terminal part of MexZ, encompassing the DNA-binding domain, is required for interaction with ArmZ. Using the bacterial two hybrid system based mutant screening and pull-down analyses we identified substitutions in MexZ that diminished (R3S, K6E, R13H) or completely impaired (K53E) the interaction with ArmZ without blocking MexZ activity as a transcriptional repressor. Introduction of corresponding mexZ missense mutations into P aeruginosa PAO1161 chromosome impaired (mexZ K6E, mexZ R13H) or blocked (mexZ K53E) tetracycline mediated induction of mexY expression. Concomitantly, PAO1161 mexZ K53E strain was more susceptible to aminoglycos...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research

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In conclusion, 3.1-PP4 is a highly promising lead towards development of a topical treatment for severely infected skin injuries.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a major public health problem. Traditionally, AMR surveillance programs for N. gonorrhoeae have focused mainly on laboratory data to describe the prevalence and trends of resistance. However, integrating individual-level risk factors (e.g. sexual orientation or international travel) with laboratory data provides important insights into factors promoting the spread of resistant N. gonorrhoeae Here, over a twelve-year period, we assessed the trends and risk factors for resistant N. gonorrhoeae in individuals attending a large publicly-funded sexual ...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
We described the emergence of the novel OXA-681 during treatment of nosocomial infection caused by a ST175 high-risk clone. The ability of OXA-681 to confer cross-resistance to ceftolozane/tazobactam and ceftazidime/avibactam together with the complex antimicrobial resistance profiles exhibited by the clinical strains harbouring this new enzyme argue for maintaining active surveillance on emerging broad-spectrum resistance in P. aeruginosa. PMID: 31383659 [PubMed - as supplied by publisher]
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Abstract Salmonella enterica serovar Goldcoast infection was rare in Taiwan; it had not been detected in routine surveillance from 2004 to 2013. This serovar was first identified in 2014 but the frequency of infection remained low until 2017. From 2014 to 2016, all but one isolate were pan-susceptible. S. Goldcoast infections abruptly increased in 2018 and all isolates were multidrug-resistant (MDR). All MDR isolates harbored an IncHI2 plasmid and the majority carried 14 antimicrobial resistance genes, including aac(3)-IId, aadA22, aph(3')-Ia, aph(6)-Id, bla TEM-1B, bla CTX-M-55, lnu(F), floR, qnrS13, arr-2, sul2,...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
In conclusion, the cross-resistance with azithromycin indicates that cethromycin may not be considered for empirical first-line monotherapy of gonorrhoea. However, cethromycin might be valuable in combination antimicrobial therapy and for second-line therapy e.g. for cases with ceftriaxone resistance or allergy. PMID: 31106713 [PubMed - as supplied by publisher]
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
Application of Antimicrobial Peptides of the Innate Immune System in Combination With Conventional Antibiotics—A Novel Way to Combat Antibiotic Resistance? Maria S. Zharkova1, Dmitriy S. Orlov1, Olga Yu. Golubeva2, Oleg B. Chakchir3, Igor E. Eliseev3, Tatyana M. Grinchuk4 and Olga V. Shamova1* 1Laboratory of Design and Synthesis of Biologically Active Peptides, Department of General Pathology and Pathophysiology, FSBSI Institute of Experimental Medicine, Saint Petersburg, Russia 2Laboratory of Nanostructures Research, Institute of Silicate Chemistry of the Russian Academy of Sciences, Saint Petersburg, Russia ...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Julija Armalytė1, Jūratė Skerniškytė1, Elena Bakienė1, Renatas Krasauskas1, Rita Šiugždinienė2, Violeta Kareivienė2, Sigita Kerzienė2, Irena Klimienė2, Edita Sužiedėlienė1 and Modestas Ružauskas2* 1Life Sciences Center, Institute of Biosciences, Vilnius University, Vilnius, Lithuania 2Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Kaunas, Lithuania Soil is one of the biggest reservoirs of microbial diversity, yet the processes that define the community dynamics are not fully understood. Apart from soil management being vital for agricultural purposes...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion: Some Lactobacillus strains exhibit anti-CRE activity, which suggests potential applications for controlling or preventing CRE colonization or infection. Introduction Although Enterobacteriaceae are normal flora of the human intestinal system, they are also common pathogens causing human infections in the setting of both community-acquired and healthcare-associated infections (Hsueh et al., 2010; Toh et al., 2012; Lai et al., 2014; Jean et al., 2016). In this era of widespread antibiotic resistance, Enterobacteriaceae are no exception. Recently, the emergence of carbapenem-resistant Enterobacteriaceae (C...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, a “species” is defined as a unique AMR profile, including the profile corresponding to susceptibility to all tested antimicrobials. The AMR profile (or antibiogram) of an isolate is the combination of AMR phenotype (susceptible or non-susceptible) to each drug tested. SR reflects the richness of AMR profiles without weighting of abundance. SE and SD are measures in which both SR and relative abundance are taken into account. The BP diversity index is related to the proportion of isolates with the most common AMR profile. Changes in antimicrobial testing protocol could impact upon results, partic...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion In this study, a self-transmissible IncN2 plasmid bearing blaNDM was detected in ST405 E. coli, which is a globally disseminated lineage. We highlight the dissemination potential of IncN2 plasmids harboring blaNDM. The presence of blaNDM mediated by plasmids in the high-risk clone ST405 E. coli linked with multiple virulence genes and resistance genes is increasing, which may create health concerns. Author Contributions YJ and ZL designed the experiments. YH analyzed the data and wrote the manuscript. CS and XG prepared the table and figures. YB carried out the bacteria identification. Funding The study was...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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