Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.
Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA. Methods Mol Biol. 2020;2069:59-78 Authors: Yamaguchi T, Ono D, Sato A Abstract Methicillin-susceptible S. aureus changes to methicillin-resistant S. aureus (MRSA) upon the acquisition of staphylococcal cassette chromosome mec (SCCmec), a genomic island that encodes methicillin resistance. SCCmec elements in S. aureus are classified into different types based on the combination of mec gene complexes and ccr gene complexes, which share variations, five classes in mec and eight in ccr. To date, at least 13 types of SCCmec elements have been identified and each SCCmec type has individual characteristics. It is known that hospital-associated MRSA strains carry SCCmec elements of types, I, II, and III, and the majority of community-acquired MRSA strains carry characteristic SCCmec elements, type IV SCCmec or type V SCCmec. We herein describe multiplex PCR methods to type SCCmec elements by identifying the mec gene complex class and ccr gene complex type. PMID: 31523765 [PubMed - in process]
ConclusionThe national screening tools were of limited use as the majority of MRSA and CPE carriers passed unidentified through the EDs, and many patients were isolated unnecessarily.
Publication date: Available online 15 October 2019Source: American Journal of Infection ControlAuthor(s): Hiroki Kitagawa, Minako Mori, Seiya Kashiyama, Yayoi Sasabe, Kiyoko Ukon, Naomi Shimokawa, Nobuaki Shime, Hiroki OhgeBackgroundThe hospital environment is an important source of multidrug-resistant organisms such as methicillin-resistant Staphylococcus aureus (MRSA). Here, we evaluated the efficacy of pulsed xenon ultraviolet (PX-UV) disinfection in addition to manual cleaning in a Japanese hospital.MethodsEnvironmental samples were collected from inpatient rooms that had been occupied for at least 48 hours by patients...
In conclusion, we found greater BMAT is associated with greater loss of trabecular bone at the spine and femoral neck, and greater loss of spine compressive strength, in older women.This article is protected by copyright. All rights reserved.
With great interest we read the article by Butler-Laporte G et al.,1 and here we reported the emergence and spread of pvl-positive genotypic CA-MRSA ST59 with increased adhesion capacity from wounds in hospitals. Methicillin-resistant Staphylococcus aureus (MRSA) has been a major pathogen causing skin and soft tissue infections (SSTIs) and invasive infections in both healthcare and community settings.1,2. Epidemiological classification and genotypic definition contribute to the understanding of healthcare-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA).
In conclusion, to best of our knowledge these findings are the first report of isolation of 4-bromophenol and Bis (2-ethylhexyl) phthalate from genus Nocardiopsis, thus suggesting that rare actinomycetes are promising source of therapeutically important bioactive metabolites.
New options are urgently needed for the treatment of methicillin-resistant Staphylococcus aureus (MRSA) infections. Balsacone C is a new dihydrochalcone extracted from Populus balsamifera that has been reported previously as being active against Staphylococcus aureus. Here, we evaluate the antibacterial activity of balsacone C against MRSA. Thirty-four (34) MRSA isolates were obtained from hospitalized patients; these isolates were then characterized for their resistance. Most of these MRSA (>85%) were resistant to penicillin, amoxicillin/clavulanic acid, ciprofloxacin, moxifloxacin, levofloxacin, clindamycin, erythromy...
Abstract We designed a few polymyxin derivatives which exhibit broad-spectrum antimicrobial activity. Lead compound P1 could disrupt bacterial membranes rapidly without developing resistance, inhibit biofilms formed by E. coli, and exhibit excellent in vivo activity in an MRSA-infected thigh burden mouse model. PMID: 31612170 [PubMed - as supplied by publisher]
In this study we demonstrate that the supernatant from novel environmental isolates of Delftia spp. have antimicrobial activity. We characterized the active fraction and identified delftibactin A as a compound with antimicrobial activity. Delftibactin A exhibits potent antimicrobial activity against Gram positive multi drug resistant (MDR) bacteria like Methicillin-resistant Staphylococcus aureus (MRSA), and Vancomycin resistant Enterococcus (VRE), and also against the Gram negative pathogens Acinetobacter baumannii and Klebsiella pneumoniae. We discovered that the production of delftibactin A is greatly influenced by temp...
Abstract The in vitro activities of five quinazolinone antibacterials, compounds Q1-Q5, were tested against 210 strains of methicillin-resistant Staphylococcus aureus (MRSA). The MIC50/MIC90 values (both in μg/ml) were as follows: Q1 (0.5/2), Q2 (1/4), Q3 (2/4), Q4 (0.06/0.25) and Q5 (0.125/0.5). Several strains with high MIC values for some of these compounds (from 8 to>32 μg/ml) exhibited amino-acid changes in the penicillin-binding proteins, which are targeted by these antibacterials. PMID: 31611358 [PubMed - as supplied by publisher]