Nested culture method improves detection of Fusobacterium from stool in patients with ulcerative colitis.

This study suggests that the recovery of F. varium is related to UC, which aligns with results from previous studies that used mucosal culture, immunostaining, real-time PCR, and serological studies. PMID: 23514906 [PubMed - in process]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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CONCLUSION:A number of studies have showed an association between PD and IBD. Both diseases share genetic and environmental etiological factors. The precise role of intestinal bacteria remains vague. The periodontal microbiota that might be involved in the association of these diseases are Fusobacterium nucleatum, Campylobacter rectus and Campylobacter concisus. Fungal and viral microbiota dysbiosis should also be evaluated as common pathogenic pathways in IBD and periodontal disease.
Source: Dental Technology Blog - Category: Dentistry Source Type: news
CONCLUSIONS: A significant dysbiosis was found in UC and CD patients compared to controls. PMID: 31293117 [PubMed - as supplied by publisher]
Source: Minerva Gastroenterologica e Dietologica - Category: Gastroenterology Tags: Minerva Gastroenterol Dietol Source Type: research
In this study, we provide new evidence that F.nucleatum accumulated heavily in the intestine of UC patients, and accompanied by the secretion of IFN-γ and the skewing of M1 macrophages. Mechanistically, our data showed that F.nucleatum aggravated dextran sodium sulfate (DSS)-induced colitis in the production of Th1-related cytokines IFN-γ through AKT2 signaling pathway in vitro and in vivo. To further confirm the disease-relevance of these shifts in macrophage repolarization in response to F.nucleatum, stimulated bone marrow derived macrophages (BMDMs) were transferred into recipient mice with DSS colitis. This...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Conclusions: A combination of fecal Fn and Fp was identified as a valuable marker for CD diagnosis. A CD bacterial marker panel may provide a simple noninvasive approach to screen for CD.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we identified 37 genus-level core bacteria from feces of 101 healthy mice with different ages, sexes, and mouse strains in three previous studies. They collectively represented nearly half of the total sequences, and predominantly included carbohydrate- and amino acids-metabolizing bacteria and immunomodulatory bacteria. Among them, Anaerostipes indwelt the gut of all healthy mice. Co-abundance analysis showed that these core genera were clustered into five groups (Group C1–C5), which were ecologically related. For example, the abundances of Group C2 including probiotics Bifidobacterium and Lactobacill...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, our study might provide evidence that GQD treats diarrhea maybe involved in modulating gut microbiota and increasing SCFA levels. Introduction Diarrheal diseases cause approximately 1.7 billion new infections and 0.76 million deaths in children of under 5 years of age annually and are thus the second most common cause of mortality in young children in developing countries (Kotloff et al., 2012, 2013). Outbreaks of such diseases are usually related to infection by common causative agents, mainly rotavirus, Salmonella enterica, and diarrheagenic Escherichia coli (Hodges and Gill, 2010; Kolenda et al., ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Na Li, Shimeng Huang, Lili Jiang, Zhaolai Dai, Tiantian Li, Dandan Han and Junjun Wang* State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China Microbial exposure during early life plays a pivotal role in modulating the health and intestinal development of the host. Our recent study showed that the low-birth-weight (LBW) piglets harbored a different fecal microbiota compared to normal-birth-weight (NBW) piglets during early life with a lower abundance of the genus Lactobacillus. Considering the spatial variations in gut microbiota at distin...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Source: Gastroenterology - Category: Gastroenterology Authors: Tags: AGA Abstracts Source Type: research
Publication date: Available online 15 February 2017 Source:The Lancet Author(s): Sudarshan Paramsothy, Michael A Kamm, Nadeem O Kaakoush, Alissa J Walsh, Johan van den Bogaerde, Douglas Samuel, Rupert W L Leong, Susan Connor, Watson Ng, Ramesh Paramsothy, Wei Xuan, Enmoore Lin, Hazel M Mitchell, Thomas J Borody Background The intestinal microbiota is implicated in the pathogenesis of ulcerative colitis. Faecal microbiota transplantation is a novel form of therapeutic microbial manipulation, but its efficacy in ulcerative colitis is uncertain. We aimed to establish the efficacy of intensive-dosing, multidonor, faecal micro...
Source: The Lancet - Category: Journals (General) Source Type: research
This study was performed to investigate the relationship between the abundance of pathogenic gut microbes in Chinese patients with inflammatory bowel disease (IBD) and disease severity. We collected clinical data and fecal samples from 47 therapy-naive Chinese patients with ulcerative colitis (UC), 67 patients with Crohn disease (CD), and 48 healthy volunteers. Bacteria levels of Fusobacterium species (spp), enterotoxigenic Bacteroides fragilis (B fragilis), enteropathogenic Escherichia coli (E coli), and Enterococcus faecalis (E faecalis) were assessed by quantitative real-time PCR (qRT-PCR). Spearman correlation coeffic...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
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