Characterization of the Escherichia coli O157:H7 Outbreak Strain Whose Shiga Toxin 2 Gene Is Inactivated by IS1203v Insertion.
Characterization of the Escherichia coli O157:H7 Outbreak Strain Whose Shiga Toxin 2 Gene Is Inactivated by IS1203v Insertion. Jpn J Infect Dis. 2013;66(3):201-6 Authors: Asano Y, Karasudani T, Tanaka H, Matsumoto J, Okada M, Nakamura K, Kondo H, Shinomiya H Abstract A total of 12 enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains were isolated during a recent outbreak in a nursery school in Ehime Prefecture, Japan. These isolates were considered to be derived from a common strain when analyzed using an IS-printing method and pulsed-field gel electrophoresis. PCR analysis revealed that the isolates harbor stx1, stx2, eae, and hlyA. However, assessment of the production of the Stx proteins revealed that these isolates produced Stx1 but not Stx2. We determined their stx2 variants such as stx2c and found that the size of the PCR product was much larger than the expected size. Sequencing of the entire stx2 gene revealed that a 1310-bp fragment was inserted into the coding region of the Stx2A subunit and that the sequences of the insert were identical to those of IS1203v. According to the sequences around the insertion site, additional amino acid residues should be attached at the C-terminus of the A subunit, which may hamper the Stx2 complex formation. Finally, this study also suggested that such an insertion may lead to the misinterpretation of results when screening EHEC isolates for virulence genes by PCR. PMID: 23698480 [PubMed - in process]
Just days after health officials urged Americans to throw out their salad kits over E. coli concerns, the Food and Drug Administration announced it's investigating three separate E. coli outbreaks linked to salad products.
Foodborne Pathogens and Disease, Ahead of Print.
CONCLUSION: the reuse of the enzymatic detergent solution is a risk to the safe processing of endoscopic devices, evidenced by its contamination with pathogenic potential microorganisms, since the enzymatic detergent has no bactericidal property and can contribute as an important source for outbreaks in patients under such procedures. PMID: 31826156 [PubMed - in process]
A dangerous new E. coli outbreak is linked to Salinas, California, a city known as the Salad Bowl of the World. At least eight people in three states have become ill. It's not clear if these cases are linked to a recent, more widespread outbreak from contaminated romaine lettuce that sickened 102 people in 23 states. Jonathan Vigliotti reports.
There are at least eight cases of E. coli in three states linked to Fresh Express Sunflower Crisp Chopped Salad Kits. But it's unclear if these new cases are connected to a more widespread outbreak from romaine lettuce. Jonathan Vigliotti reports.
It's unclear if these new cases are linked to a more widespread outbreak from romaine
This outbreak is caused by a different strain of E. coli than the other current outbreak linked to romaine lettuce grown in the Salinas, California region, which has over 100 reported cases of infection.
As part of the ongoing investigation into the multistate outbreak of Escherichia coli O157:H7 ( E. coli O157) infections, Wisconsin health and food safety officials have found E. coli O157:H7 bacteria in an unopened bag of pre-washed chopped romaine collected from an ill person ’s home. Additional laboratory testing is pending to determine if the E. coli O157 found in the pre-washed chopped romaine matches the strain causing the multi-state outbreak linked to romaine lettuce. The E. coli O157...(see release)
The FDA, CDC, state health authorities, and Canadian officials have been investigating an E. coli illness outbreak, and research shows that Fresh Express brand Sunflower Crisp Chopped Salad Kits are the likely source.
TUESDAY, Dec. 10, 2019 -- The likely source of an E. coli outbreak that has sickened eight people in three states is Fresh Express Sunflower Crisp Chopped Salad Kits, the U.S. Food and Drug Administration said Monday. As of Dec. 9, there have been...