Perfluorooctanoic acid induces migration and invasion and inhibits apoptosis through the PI3K/AKT signaling pathway in human rhabdomyosarcoma cells.

Perfluorooctanoic acid induces migration and invasion and inhibits apoptosis through the PI3K/AKT signaling pathway in human rhabdomyosarcoma cells. Oncol Rep. 2019 Aug 06;: Authors: Zhang Q, Wang J, Chen C, Kong Y, Yan H, Duan J, Wang C, Sha Y, Wen X, Wang C Abstract The present study aimed to investigate the effects of perfluorooctanoic acid (PFOA) on tumor cell migration, invasion and apoptosis by activation of the PI3K/AKT signaling pathway in human rhabdomyosarcoma (RMS). PFOA is a persistent, synthetic organic environment pollutant, which has been previously associated with multiple diseases, including cancer. The present study aimed to confirm whether PFOA can elicit cell growth in the RD subline of RBS. RD cells were treated with different concentrations of PFOA. Cell proliferation was evaluated using Cell Counting Kit‑8 and cell cycle assays. Cell migration and invasion were determined using wound healing and Transwell assays. Apoptotic rates were estimated by Annexin V‑FITC/propidium iodide staining. The expression levels of vimentin, serum/glucocorticoid‑regulated kinase 1 (SGK1), cyclin E2, cyclin dependent kinase (CDK)2, p53, p21, p27, phosphatidylinositol‑3 kinase (PI3K) and protein kinase B (AKT), and apoptosis‑associated genes and proteins (including Bcl‑2 and Bax) were detected by reverse transcription‑PCR and western blot analyses. The results showed that PFOA significantly promoted RD cell proliferat...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research