Expression and significance of Annexin A3 in the osteosarcoma cell lines HOS and U2OS.

Expression and significance of Annexin A3 in the osteosarcoma cell lines HOS and U2OS. Mol Med Rep. 2019 Jul 23;: Authors: Zeng X, Wang S, Gui P, Wu H, Li Z Abstract Annexin A3 (ANXA3) is highly expressed in different types of cancers, but the impact of ANXA3 in bone tumors is still not clear. In the present study, the expression of ANXA3 in osteosarcoma cells was first confirmed by cellular immunofluorescence. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis were used to detect the expression of ANXA3 in osteoblasts in the osteosarcoma cell lines U2OS and HOS. Furthermore, small interfering (si)‑RNA were transfected into U2OS and HOS cells via a liposome‑mediated method. Then once ANXA3 had been successfully downregulated in U2OS and HOS cells, the cells were collected and total protein was extracted after 48 h of transfection. Western blot analysis was used to confirm successful ANXA3 transfection into osteosarcoma cells and the apoptotic rate of HOS and U2OS was detected by flow cytometry. The expression of ANXA3 in the osteosarcoma cell lines HOS and U2OS were first observed by confocal laser scanning microscopy, and was then detected by RT‑qPCR and western blotting. The mRNA and protein levels of ANXA3 in the osteosarcoma cell lines HOS and U2OS were significantly increased compared with osteoblasts, particularly in HOS cells. When siRNA was transfected into HOS and U2OS cells, the protei...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research

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ConclusionsThe combination of ALRN-6924 and palbociclib at pharmacologically relevant doses is feasible and generally well tolerated.Clinical trial identificationNCT02909972.Legal entity responsible for the studyAileron Therapeutics, Inc.FundingAileron Therapeutics, Inc.DisclosureF. Meric-Bernstam: Honoraria (self): Sumitomo Dainippon Pharma; Dialectica; Advisory / Consultancy: Genentech; Advisory / Consultancy: Inflection Biosciences; Advisory / Consultancy: Pieris; Advisory / Consultancy: Darwin Health; Advisory / Consultancy: Samsung bioepis; Advisory / Consultancy: Aduro; Advisory / Consultancy: Spectrum; Advisory / Co...
Source: Annals of Oncology - Category: Cancer & Oncology Source Type: research
Conclusions: Preoperative CT examination omits a few small pulmonary metastases of osteosarcoma and there is rare progress in recent years. Therefore, we recommend that patients with pulmonary metastases of undergo thoracotomy to locate and resect all metastases as much as possible through intraoperative direct palpation. PMID: 31559064 [PubMed]
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Conclusions: This case highlights the importance of timely consideration of osteosarcoma in an infant, when the clinical presentation and medical imaging are consistent with that diagnosis.
Source: Journal of Pediatric Hematology Oncology - Category: Hematology Tags: Clinical and Laboratory Observations Source Type: research
Currently, the selenium-doped hydroxyapatite (Se-HAp) nanoparticles have received immense therapeutic and tissue repairing values owing to their antitumor activities. However, a high concentration of Se is toxic towards normal and stem cells whereas its low concentration cannot effectively remove the cancer cells. Therefore, the current study was aimed to improve the anticancer activity of Se-HAp nanoparticles through catechins (CC) modification owing to their high cancer therapeutic value. The sequentially developed catechins modified Se-HAp nanocomposites (CC/Se-HAp) were characterized for various physicochemical propert...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Sodium fluoride (NaF) PET/CT rarely adds value for detecting bone metastases...Read more on AuntMinnie.comRelated Reading: Could Ga-68 PSMA PET become essential for prostate cancer? Delayed NaF-PET enhances coronary plaque detection Can deep learning monitor lesions on F-18 NaF PET/CT? CMS again declines coverage for NaF-PET scans NaF-PET could be valuable for osteosarcoma assessment
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
Discussion: A deep leaning network was trained to segment sarcomas on pediatric patients. Sarcomas are a very heterogenous class of tumors with large variations in size, shape and metabolism. This showed to be a challenging problem. Nevertheless, the network was able to accurately segment quite complex tumors (See figure, automatic segmentation in red outline) while in other cases it would fail. We attribute this to the limited number of training cases and hypothesize that an increasing number of training samples would improve the network. References: [1] Ronneberger et al, U-Net: Convolutional Networks for Biomedical Imag...
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Data Sciences Posters Source Type: research
CONCLUSIONS: At the moment there is no evidence from individual studies in children with osteosarcoma or hepatoblastoma treated with different platinum analogues and dosage schedules that underscores the use of amifostine as an otoprotective intervention as compared to no additional treatment. Since pooling of results was not possible and the evidence was of low certainty, no definitive conclusions can be made. Since we found only one RCT evaluating the use of sodium thiosulfate in children with localized hepatoblastoma treated with cisplatin, no definitive conclusions on benefits and harms can be drawn. It should be noted...
Source: Cochrane Database of Systematic Reviews - Category: General Medicine Authors: Tags: Cochrane Database Syst Rev Source Type: research
Conclusions: The proposed nomogram is a reliable and robust tool for accurate prognostic prediction in patients with extremity soft tissue LMS. Introduction Soft tissue leiomyosarcoma (LMS) is an aggressive sarcoma, which originates from smooth muscle cells (1). Soft tissue LMS accounts for about 5–10% of all soft tissue sarcomas (2). It occurs in different sites, including the retroperitoneum, intraabdominal sites, and extremities (3). Extremity LMS comprised about 10–15% of extremity sarcomas, with a preference for the lower limb (4–6). Extremity LMS tends to have a better prognosis than uterine...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Conclusions Several model systems are now available to characterize the MSC-tumour interplay in the TME. These offer early promise in establishing robust preclinical platforms for the identification of crucial molecular pathways and for the assessment of clinical efficacy of novel drugs to inhibit cancer development and progression. However, selection of the right model for a given study should be shaped on the purpose, and should also consider fixed biological, biochemical, and biophysical parameters according to the specific tumour type. Finally, in order to get reliable and useful results to be translated to the clinic...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Conclusion: Fat fraction on MRI corresponded to fat content on histology and therefore contributes to lesion characterization. Measurement repeatability was excellent for ADC; this parameter increased significantly post-radiotherapy even in disease categorized as stable by size criteria, and corresponded to cellularity on histology. ADC can be utilized for characterizing and assessing response in heterogeneous retroperitoneal sarcomas. Introduction Soft-tissue sarcomas are often highly heterogeneous tumors with variable components that can include cellular tumor, fat, necrosis, and cystic change. In many soft-tissue ...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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