Effects and mechanisms of iridoid glycosides from Patrinia scabiosaefolia on improving insulin resistance in 3T3-L1 adipocytes.

Effects and mechanisms of iridoid glycosides from Patrinia scabiosaefolia on improving insulin resistance in 3T3-L1 adipocytes. Food Chem Toxicol. 2019 Sep 12;:110806 Authors: Liu Z, Xu L, Xu X, Niu Y, Saadeldeen FSA, Kang W Abstract Insulin resistance causes several adverse effects such as hypertension, diabetes and different aspects of cardiovascular diseases. Patrinia scabiosaefolia Fisch. ex Trev. is a traditional Chinese edible herbal, whose n-BuOH extract significantly increased glucose transport in 3T3-L1 adipocytes at 12.5 μM. Further analysis lead to find two compounds (1-2) with significant activity on improving insulin resistance in 3T3-L1 adipocytes at concentrations around 25.0 μM (P < 0.001). Compound 2 was more effective by lowering glucose content at 12.5 μM (P < 0.001). Compound 1 represents a new compound identified by spectroscopic methods. Western-blot experiment demonstrated an upregulation of p-IRS-1, p-Akt, and GLUT4 induced by compounds 1 and 2. Hence, we speculate that compounds 1 and 2 activate PI3K and Akt signaling through up-regulating of p-IRS-1 which results in the activation of PI3K before phosphorylating Akt, ultimately leading to translocation of GLUT4. These events finally improve glucose transport. Our results may provide the scientific basis for the development and effective use of P. scabiosaefolia against type 2 diabetes. PMID: 31521635 [PubMed - as supplied by pub...
Source: Food and Chemical Toxicology - Category: Food Science Authors: Tags: Food Chem Toxicol Source Type: research