IJERPH, Vol. 16, Pages 3439: Antibiotic Susceptibility Patterns, Biofilm Formation and esp Gene among Clinical Enterococci: Is There Any Association?

IJERPH, Vol. 16, Pages 3439: Antibiotic Susceptibility Patterns, Biofilm Formation and esp Gene among Clinical Enterococci: Is There Any Association? International Journal of Environmental Research and Public Health doi: 10.3390/ijerph16183439 Authors: Poh Leng Weng Ramliza Ramli Rukman Awang Hamat Enterococci are commonly found in humans, animals and environments. Their highly adaptive mechanisms are related to several virulent determinants and their ability to resist antibiotics. Data on the relationship between the esp gene, biofilm formation and antibiotic susceptibility profiles may differ between countries. This cross-sectional study was conducted to determine the proportion of esp gene and biofilm formation among Enterococcus faecalis and Enterococcus faecium clinical isolates. We also investigated the possible association between the esp gene with antibiotic susceptibility patterns and biofilm formation. The isolates were collected from clinical samples and identified using biochemical tests and 16SRNA. Antibiotic susceptibility patterns and a biofilm assay were conducted according to the established guidelines. Molecular detection by PCR was used to identify the esp gene using established primers. In total, 52 and 28 of E. faecalis and E. faecium were identified, respectively. E. faecium exhibited higher resistance rates compared to E. faecalis as follows: piperacillin/tazobactam (100% versus 1.9%), ampicillin (92.8% versus 1.9%), high-level gentamicin re...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research

Related Links:

Abstract Multidrug resistance is one of the top three threats to global public health. Understanding resistance of bacteria is important to help decrease resistance and improve the development of novel antimicrobial agents or other alternative tools to combat public health challenges. Thus, the goal of this study was to investigate the vancomycin and florfenicol resistance genes of five E. faecalis and 15 E. faecium isolated from patients with urinary tract infections. There were 20 Enterococcus obtained from the library collection of randomly selected private hospitals located in the city of El Qanater El Khayrey...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research
This study aimed to evaluate a cumulative antimicrobial resistance index (ARI) as a possible key outcome measure of antimicrobial stewardship programmes (ASPs) and a tool to predict the antimicrobial resistance (AMR) trend.MethodsAntimicrobial susceptibility for Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. and Escherichia coli (ESKAPEEc) pathogens recovered from blood cultures during a 5-year period (2014–2018) was analysed to obtain a cumulative ARI. For each antibiotic tested, a score of 0, 0.5 or 1 was assigned for susceptibi...
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Abstract Mastitis caused by multi- or pan-drug resistant bacteria is a growing health concern. A total of 110 milk samples were collected: Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Enterococcus faecalis, and Escherichia coli were present in 54/110 (49.09%), 37/110 (33.63%), 25/110 (22.72%), 7/110 (6.36%), and 50/110 (45.45%) samples, respectively. A total of 20 methicillin-resistant S. aureus (MRSA) isolates, 19 Streptococcus sp. isolates, and 15 E. coli isolates were selected, and 100% were positive for (coagulase and hemolysins), streptokinase, and hemolytic activity, respectiv...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
ConclusionMultidrug resistance was higher among HLGR and HLSR isolates compared to non-HLGR and non-HLSR isolates which is a concern because it results in limitation of treatment choices. More than 50% of the isolates were sensitive to aminoglycosides; hence correct identification in clinical laboratories and administration of these antibiotics can result in decrease of antibiotics such as Vancomycin and Linezolid and help to reduce the emergence of resistance to these drugs.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionsWGS proved to be an effective tool to differentiate several LREfm clusters in an outbreak. Active screening for LREfm is important in a high-risk setting such as hematooncology, where multiple introductions are possible and occur despite intensified infection control measures.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
ConclusionsEnterococcal pRE25-like elements are suspected to be widespread in MDRSP isolated from companion dogs in Korea. Considering that companion dogs live in a closely shared environment with humans, continuous surveillance of pRE25-like elements is needed for other staphylococci commonly isolated from humans.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
This study analyzed Escherichia coli (E. coli) and Enterococcus spp. from vegetables and soil in three urban community gardens in metro Detroit, Michigan. Bacteria were identified by PCR and 16S rRNA sequencing, followed by antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing (WGS). E. coli was isolated from 11 of 19 (57.9%) soil samples and 23 of 48 (48%) vegetables, whereas Enterococcus spp. from 17 of 19 (89.5%) soils and 38 of 48 (79%) vegetables. The most common Enterococcus species identified in soil were Ent. mundtii (29.4%), Ent. casseliflavus (11.8%), Ent. dura...
Source: Food Control - Category: Food Science Source Type: research
ConclusionThe proportion of VREF is increasing in German hospitals, particularly in southern regions in Germany. Increased efforts in infection control and antibiotic stewardship activities accounting for local resistance patterns are necessary to combat the spread of VREF in Germany.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Publication date: September 2019Source: Journal of Global Antimicrobial Resistance, Volume 18Author(s): Tanya Strateva, Slavil Peykov, Ivo Sirakov, Encho Savov, Svetoslav Dimov, Ivan Mitov
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
ConclusionsOur finding demonstrated that the fosB gene, mutations in the fosfomycin target enzyme MurA, and a high expression level of fosX were the resistance mechanisms in these enterococci.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
More News: Antimicrobial Resistance | Enterococcus | Environmental Health | Genetics | Hospitals | International Medicine & Public Health | Penicillin | Statistics | Study