Rapid culture-based identification of Shiga toxin-producing Escherichia coli and Shigella spp./Enteroinvasive E. coli using the eazyplex ® EHEC complete assay

This study shows that eazyplex® EHEC is a very fast and easy to perform molecular assay that provides reliable results as a culture confirmation assay for the diagnosis of STEC andShigella spp./EIEC infections.
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research

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Conclusion: Specific standards should be kept to prevent outbreaks during athletic events. This was the first time that a foodborne outbreak due to Clostridium perfringens was investigated in the country. Laboratory investigation for toxins should be enhanced, especially in foodborne outbreaks where clinical manifestations of cases are found to be compatible with infection caused by a toxin.
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
A sunburn may not be the only health consequence of your summer beach trips and pool days. A study published last year estimated that recreational water activities are related to 90 million illnesses each year in the U.S., with swimming as one of the primary catalysts of water-borne respiratory, ear and skin infections. Less frequently, according to federal data, pathogens found in pools, lakes, rivers and oceans can lead to more serious sickness, including gastrointestinal illnesses and—in very rare cases—exposure to flesh-eating bacteria. This month, for example, a Florida woman died from a flesh-eating bacte...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Infectious Disease Source Type: news
A recent announcement from the U.S. Centers for Disease Control and Prevention (CDC) may put a damper on summer fun. A fecal parasite often spread by swimming is causing an increasing number of illness outbreaks, the agency says. The number of disease outbreaks involving the parasite Cryptosporidium, also known as Crypto, increased by about 13% each year from 2009 to 2017, according to a new report from the CDC. People can become ill with cryptosporidiosis after exposure to contaminated human or animal fecal matter, developing symptoms including nausea, cramps and diarrhea that can last weeks and lead to serious malnutriti...
Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized public health Source Type: news
CONCLUSION: The occurrence of genetically similar diarrheagenic E. coli in both groups of children, likewise resistant to these agents, underscores the importance of establishing strategies for the prevention of outbreaks, especially among low-income households. PMID: 31199225 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
PMID: 31048673 [PubMed - in process]
Source: MMWR Morb Mortal Wkl... - Category: Epidemiology Authors: Tags: MMWR Morb Mortal Wkly Rep Source Type: research
Conclusion Since B. cereus group are highly similar in biochemical as well as genetic profiles, a new biomarker was developed for identifying and distinguishing B. thuringiensis from the closely related group. The performance of XRE was compared with cry2 gene and artificially contaminated samples were also tested. Compared with cry2, XRE gene was observed to be efficiently accurate in the identification of B. thuringiensis. Further, the developed real-time PCR using XRE successfully identified B. thuringiensis and it could be used to quantify cell numbers with the generated standard curve. Author Contributions The expe...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
The objective of this research was to determine changes to lettuce phyllosphere microbiota, inoculated ARB, and the resistome (profile of ARGs) following washing with a sanitizer, gamma irradiation, and cold storage. To simulate potential sources of pre-harvest contamination, romaine lettuce leaves were inoculated with compost slurry containing antibiotic-resistant strains of pathogenic (Escherichia coli O157:H7) and representative of spoilage bacteria (Pseudomonas aeruginosa). Various combinations of washing with sodium hypochlorite (50 ppm free chlorine), packaging under modified atmosphere (98% nitrogen), irradiating (1...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
ConclusionvB-SdyS-ISF003 is a novel virulent T1virus phage and has the appropriate potential for being used in bio controlling of S. dysenteriae in different condition.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
AbstractA gastroenteritis outbreak occurred in a university in May, 2017, Wuhan, China. The epidemiological survey and pathogen analysis were conducted to identify the pathogen and control this outbreak. Feces or anal swabs from individuals, water, and swabs taken from tap surfaces of the secondary water supply system (SWSS) and foods were collected for the detection of viruses and pathogenic enteric bacteria by real-time RT-PCR and culture, respectively. Nucleotide sequences were determined by RT-PCR and direct sequencing. Genotyping, phylogenetic, and recombination analyses were conducted by a web-based genotyping tool, ...
Source: Food and Environmental Virology - Category: Virology Source Type: research
S, Bidet P Abstract We developed a multiplex PCR method based on Multiple-Locus Variable-number of tandem repeats (VNTR)-Analysis (MLVA) designed for rapid typing of E. coli and Shigella isolates The method amplifying seven VNTRs doesn't require sequencing capillary nor fluorescent dyes. Amplification products are simply loaded on a standard agarose gel for electrophoresis and banding patterns are analyzed visually. We evaluated the method on 220 strains belonging to different collections: ECOR collection (n=72), O1:K1 isolates causing neonatal meningitis (n=38), extended-spectrum beta-lactamase-producing fecal i...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
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