Influence of fiber architecture and growth factor formulation on osteoblastic differentiation of mesenchymal stem cells in coacervate-coated electrospun fibrous scaffolds

In this study, we investigated the influence of structure of extracellular matrix-mimicking electrospun poly (l-lactic acid) (PLLA) fibrous scaffolds and incorporated growth factors (bone morphogenetic protein2 (BMP2) and platelet-derived growth factor (PDGF)) (GFs) on in vitro differentiation of human mesenchymal stem cells (hMSCs) cultured onto the scaffolds. Furthermore, Cargo GFs were first encapsulated into coacervate (Coa). Coa-coated nano-sized PLLA (Coa-nPLLA) exhibited relatively higher coating efficacy, than Coa-coated micro-sized PLLA (Coa-mPLLA) due to large mesh hole area of mPLLA. Consequently, a faster GF release pattern was observed in nPLLA groups. As a result of osteogenic differentiation of hMSC which cultured onto GFs loaded Coa-PLLA, the architectural cue of fiber diameters effectively modulated early osteogenic differentiation of hMSCs. Facilitated long-term differentiation of hMSCs could be achieved by synergistic effect of dual BMP2 and PDGF in nanofbrous environments.Graphical abstract
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research

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CONCLUSIONS: Apoptosis is an important clinical target to protect NSCs during brain tumor radiotherapy using carbon-ion radiation as well as conventional X-rays. PMID: 31633435 [PubMed - as supplied by publisher]
Source: International Journal of Radiation Biology - Category: Radiology Tags: Int J Radiat Biol Source Type: research
Rancho Cordova-based Cesca Therapeutics Inc. says it's launching a joint venture to create a blood bank-style service for immune cells. Cesca (NASDAQ: KOOL) announced its joint venture with Irvine-based HealthBanks Biotech USA Inc. on Tuesday. Both companies are affiliated with Boyalife Group Inc., a Chinese group of stem cell companies. Boyalife bought a 70% stake in Cesca in 2016, and HealthBanks is a subsidiary. The joint vent ure plans to create a facility that would be “among the first immune…
Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
The transcription factor B cell CLL/lymphoma 11B (BCL11B) is indispensable for T lineage development of lymphoid progenitors. Here, we show that chimeric antigen receptor (CAR) expression during early phases of ex vivo generation of lymphoid progenitors suppressed BCL11B, leading to suppression of T cell–associated gene expression and acquisition of NK cell–like properties. Upon adoptive transfer into hematopoietic stem cell transplant recipients, CAR-expressing lymphoid progenitors differentiated into CAR-induced killer (CARiK) cells that mediated potent antigen-directed antileukemic activity even across MHC b...
Source: Journal of Clinical Investigation - Category: Biomedical Science Authors: Source Type: research
In conclusion, these results reveal that PIK3CA is significantly downregulated by miR-27b expression in CSCs. Thus, we presume that miR-27b may be a therapeutic anti-tumor agent for CRC via targeting PI3K p110α. PMID: 31632566 [PubMed]
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
In this study, a mouse model of chronic alcoholic hepatitis was established by eight weeks of Lieber-DeCarli liquid diet feeding; and LGG-s, BMMSCs or a combination of the two were used to explore a new therapeutic method for alcoholic liver disease and to study the mechanism. The results showed that the combined LGG-s and BMMSC treatment might have a synergistic effect and could improve the symptoms of alcoholic hepatitis by regulating inflammation, autophagy and lymphocyte subsets through the PI3k/NF-kB and PI3K/mTOR pathways. With the treatment, the autophagy rate accelerated, and alcohol-induced natural killer B (NKB) ...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
This study aims to investigate the effects of Tianqijiangtang capsule on the survival, self-renewal and differentiation of hippocampal neural stem cells (NSCs) of embryonic rats cultured in high glucose medium. MATERIALS AND METHODS: A cell model of diabetic encephalopathy was established. Cell viability was assessed to screen the optimal concentration of glucose for the cell model of diabetic encephalopathy. Then, the effects of Tianqijiangtang capsule on the proliferation and differentiation of NSCs, and the expression of vascular endothelial growth factor (VEGF) and brain-derived neurotrophic factor (BDNF) in the cu...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Authors: Ding X, Yang L, Hu Y, Yu J, Tang Y, Luo D, Zheng L Abstract Type 2 diabetes mellitus (T2DM), a leading cause of osteoporosis, remains a contraindication for bone implant therapy. Although associated with side effects when systemically administered, biphosphonates (BPs) play a positive role in diabetic osteoporosis treatment. We hypothesized that local BP therapy would prevent decayed implant osseointegration under T2DM conditions. To assess cell proliferation and determine the optimal BP concentration, bone marrow-derived mesenchymal stem cells (BMSCs) and bone marrow macrophages (BMMs) were treated with B...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Authors: Xu Y, Li Y, Liu Y, Li H, Jia Z, Tang Y, Jiang G, Zhang X, Duan L Abstract The repair of long-segment tracheal defects remains a significant clinical challenge, to which, optimal biologically functioning tracheal alternatives may serve as a solution. Tissue-engineered trachea, regenerated from a decellularized trachea matrix using the laser micropore technique (LDTM), demonstrates the possibility of developing optimal tracheal substitutes, which retain the original tubular shape and adequate cartilage regeneration ability of trachea. However, the strict requirement with respect to the implantation cell dens...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
This study tested the hypothesis that early administration with cold water (CW)-assisted adipose-derived mesenchymal stem cell (ADMSC)-derived exosome (Exo) therapy was superior to either one on protecting the heart against ischemia-reperfusion (IR) (i.e., by ligation of 50 minutes and relieved by day 5 prior to euthanizing the animals) injury. Adult-male SD rats (n=30) were equally categorized into groups 1 (sham-operated control), 2 (IR), 3 (IR + CW), 4 (IR + Exo) and 5 (IR + CW-Exo). The left ventricular ejection fraction (LVEF) was highest in group 1, lowest in group 2, and significantly higher in group 5 than in group...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
Authors: Chen KH, Lin KC, Wallace CG, Li YC, Shao PL, Chiang JY, Sung PH, Yip HK Abstract We tested the hypothesis that human induced pluripotent stem cell-derived mesenchymal stem cell (iPSC-MSC) therapy could effectively reduce brain-infarct volume (BIV) and improve neurological function in rat after acute intracranial hemorrhage (ICH) induced by a weight-drop device. Adult-male SD rats (n=40) were equally divided into group 1 (sham-operated control), group 2 (ICH), group 3 (ICH + hyaluronic acid (HA)/intracranial injection at 3 h after ICH), group 4 [ICH + HA + iPSC-MSC (1.2 × 106 cells/intracranial inject...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research
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