Genes, Vol. 10, Pages 716: Expression of ZNF695 Transcript Variants in Childhood B-Cell Acute Lymphoblastic Leukemia

Genes, Vol. 10, Pages 716: Expression of ZNF695 Transcript Variants in Childhood B-Cell Acute Lymphoblastic Leukemia Genes doi: 10.3390/genes10090716 Authors: Rosa Villegas-Ruíz Caballero-Palacios Pérez-López Murata Zapata-Tarres Cárdenas-Cardos Paredes-Aguilera Rivera-Luna Juárez-Méndez B-cell acute lymphoblastic leukemia is the most commonly diagnosed childhood malignancy worldwide; more than 50% of these cases are diagnosed in Mexico. Although the five-year survival rate is >80%, 30% of patients experience relapse with poor prognosis. Cancer-associated gene expression profiles have been identified in several malignancies, and some transcripts have been used to predict disease prognosis. The human transcriptome is incompletely elucidated; moreover, more than 80% of transcripts can be processed via alternative splicing (AS), which increases transcript and protein diversity. The human transcriptome is divided; coding RNA accounts for 2%, and the remaining 98% is noncoding RNA. Noncoding RNA can undergo AS, promoting the diversity of noncoding transcripts. We designed specific primers to amplify previously reported alternative transcript variants of ZNF695 and showed that six ZNF695 transcript variants are co-expressed in cancer cell lines. The amplicons were sequenced and identified. Additionally, we analyzed the expression of these six transcript variants in bone marrow from B-cell acute lymphoblastic leukemia patients and...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Article Source Type: research