Genes, Vol. 10, Pages 716: Expression of ZNF695 Transcript Variants in Childhood B-Cell Acute Lymphoblastic Leukemia
Genes, Vol. 10, Pages 716: Expression of ZNF695 Transcript Variants in Childhood B-Cell Acute Lymphoblastic Leukemia
Genes doi: 10.3390/genes10090716
Authors:
Rosa
Villegas-Ruíz
Caballero-Palacios
Pérez-López
Murata
Zapata-Tarres
Cárdenas-Cardos
Paredes-Aguilera
Rivera-Luna
Juárez-Méndez
B-cell acute lymphoblastic leukemia is the most commonly diagnosed childhood malignancy worldwide; more than 50% of these cases are diagnosed in Mexico. Although the five-year survival rate is >80%, 30% of patients experience relapse with poor prognosis. Cancer-associated gene expression profiles have been identified in several malignancies, and some transcripts have been used to predict disease prognosis. The human transcriptome is incompletely elucidated; moreover, more than 80% of transcripts can be processed via alternative splicing (AS), which increases transcript and protein diversity. The human transcriptome is divided; coding RNA accounts for 2%, and the remaining 98% is noncoding RNA. Noncoding RNA can undergo AS, promoting the diversity of noncoding transcripts. We designed specific primers to amplify previously reported alternative transcript variants of ZNF695 and showed that six ZNF695 transcript variants are co-expressed in cancer cell lines. The amplicons were sequenced and identified. Additionally, we analyzed the expression of these six transcript variants in bone marrow from B-cell acute lymphoblastic leukemia patients and...
Source: Genes - Category: Genetics & Stem Cells Authors: Rosa Villegas-Ru íz Caballero-Palacios P érez-López Murata Zapata-Tarres C árdenas-Cardos Paredes-Aguilera Rivera-Luna Ju árez-Méndez Tags: Article Source Type: research
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