Lymphatic Cannulation for Lymph Sampling and Molecular Delivery.

In this study, we describe a protocol for cannulation of mouse and rat lymphatic collectors that is suitable for the following: the "omic" sampling of pre- and postnodal lymph, collected from different anatomical districts; the phenotyping of immune cells circulating between parenchymal organs and draining lymph nodes; injection of known amounts of molecules for quantitative immunological studies of nodal trafficking and/or clearance; and monitoring an organ's biochemical omic changes in pathological conditions. Our data indicate that probing the lymphatic fluid can provide an accurate snapshot of an organ's physiology/pathology, making it an ideal target for liquid biopsy.
PMID: 31519866 [PubMed - as supplied by publisher]

Source: Journal of Immunology - September 12, 2019 Category: Allergy & Immunology Authors: Zawieja DC, Thangaswamy S, Wang W, Furtado R, Clement CC, Papadopoulos Z, Vigano M, Bridenbaugh EA, Zolla L, Gashev AA, Kipnis J, Lauvau G, Santambrogio L Tags: J Immunol Source Type: research