Microextraction of aromatic microbial metabolites by packed hypercrosslinked polystyrene from blood serum

Publication date: Available online 14 September 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Alisa K. Pautova, Pavel D. Sobolev, Alexander I. RevelskyAbstractThe article is devoted to the application of modern sample preparation technique - microextraction by packed sorbent (MEPS) - in conjunction with non-conventional type of sorbent - hypercrosslinked polystyrene, that was investigated for the first time in this work. Their combination was used to extract phenylcarboxylic acid-type aromatic microbial metabolites from serum samples of a healthy volunteer with following derivatization and GC-MS detection. As barrel insert and needle for MEPS with hypercrosslinked polystyrene is not produced, we designed a device to imitate the commercial MEPS system with packed granular biporous hypercrosslinked polystyrene. Nine aromatic microbial metabolites, including sepsis associated phenyllactic, 4-hydroxyphenyllactic and 4-hydroxyphenylacetic acids, were extracted from serum samples (recoveries were 20-70%) and a linear dependence was revealed in the most clinically significant range of concentrations (0.5-18 µM). The results obtained demonstrate the perspective of the applying of hypercrosslinked polystyrene in commercial devices for MEPS for the future analyses of biological samples, in particular for the early diagnosis of sepsis and treatment effectiveness control.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

Related Links:

Authors: Hanewinkel R PMID: 31623003 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Zum Kommentar von Prof. Reiner Hanewinkel zum Hot Topic-Thema „Raucherentwöhnung: E-Zigarette besser als klassische Nikotinersatzpräparate?“ Pneumologie 2019; 73: 336 – 337. Pneumologie. 2019 Oct;73(10):622 Authors: Pommer P PMID: 31623002 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Schulz H, Karrasch S, Bölke G, Cyrys J, Hornberg C, Pickford R, Schneider A, Witt C, Hoffmann B PMID: 31623001 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Leserbrief zu Schulz H, Karrasch S, Bölke G et al. Atmen: Luftschadstoffe und Gesundheit – Teil I. Pneumologie 2019; 73: 288 – 305. Pneumologie. 2019 Oct;73(10):617-619 Authors: Schnitzler A PMID: 31623000 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Schöll N, Rohde GGU Abstract Pneumonia belongs to the most frequent and most deadly infectious diseases worldwide. It represents an increasing problem for the aging population. The incidence and mortality rises with every decade. The clinical presentation of pneumonia differs between elderly and younger patients. Multiple factors including functional status (self-dependency and immobilization), comorbidities, immunosenescence, nutritional status, swallowing disorders have to be accounted for. Pneumonia in the elderly has to be differentiated from nursing home acquired pneumonia. Diagnosis of pneumonia...
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Perikardpunktion – Schritt für Schritt. Pneumologie. 2019 Oct;73(10):597-604 Authors: Ferrari MW PMID: 31622998 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Raspe M, Rolling T, Leisse C, Fischer J, Lehmann C Abstract Infectious Diseases are a cross-sectional area connected to various medical disciplines and offer interested physicians multiple working opportunities. The spectrum of infectious diseases covers both out- and inpatient care as well as basic, clinical and epidemiological research. The need for infectious diseases specialists is increasing, thus career prospects are promising. Working conditions in infectious diseases are comparatively family-friendly. With this article we intend to arouse interest for working in the fascinating fields of infectious...
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Authors: Bahmer T, Wälscher J, Fisser C, Kreuter M, Karg O, Böing S, Koczulla R, Raspe M PMID: 31622996 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
Lungengesundheit: Beruf als großer Risikofaktor? Pneumologie. 2019 Sep;73(9):e2 Authors: Simon A PMID: 31622984 [PubMed - in process]
Source: Pneumologie - Category: Respiratory Medicine Tags: Pneumologie Source Type: research
20 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: Am J Trop Med Hyg These pubmed results were generated on 2019/10/19PubMed comprises more than millions of citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Source: Am J Trop Med Hyg - Category: Infectious Diseases Tags: Report Source Type: research
More News: Drugs & Pharmacology