Research and Application of Glycoprotein Sensors Based on Glycosyl Recognition

Publication date: September 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 9Author(s): Jun-Yu LIANG, Pei-Hong TONG, Jian-Ping LIAbstractGlycoprotein is a binding protein whose structure is mainly determined by the content of sugars and proteins and the peptide bonds to which it binds. There are many kinds of glycoproteins, and they can be classified into lectin, hormone, enzyme and immunoglobulin according to the clinical role in the medical field. Glycoproteins have a variety of biological functions and are of great significance in the medical and biological fields. In order to study the biological information related to glycoproteins, it is necessary to establish a highly sensitive and efficient analytical detection method for glycoproteins. The assay of glycoproteins includes separation and purification method, structural analysis and content determination. The commonly used methods for glycoproteins determination are mass spectrometry (MS), chromatography-mass spectrometry, surface excimer resonance, and enzyme-linked immunosorbent assay (ELISA), etc. In recent years, in the detection of glycoproteins, the research and application of sensors have gradually increased. This paper briefly summarized the analysis methods of glycoproteins and reviewed the research and application of glycoprotein biosensors based on glycosyl recognition. The glycoprotein biosensors including electrochemical sensors, optical sensors and mass sensors were introduced in deta...
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research