New Methods for Cell Cycle Analysis

Publication date: September 2019Source: Chinese Journal of Analytical Chemistry, Volume 47, Issue 9Author(s): Han-Shu FANG, Ming-Fei LANG, Jing SUNAbstractCell cycle is a basic process during the cell life. Analyzing cell cycles can better understand the nature of diseases. Currently, cell cycles are mainly analyzed by flow cytometry to investigate intracellular DNA contents and cell cycle specific proteins. With the development of technology, several new methods have emerged for cell cycle analysis in recent years to improve and enhance image analysis, single-cell analysis, sample reduction, and high throughput. These new methods include image flow cytometry, mass flow cytometry, microfluidic technology, and Raman spectroscopy, which have greatly expanded the methods for cell cycle analysis and increased the accuracy of cell cycle measurements. Here we briefly introduce these new methods for cell cycle analysis.Graphical abstractNew methods for cell cycle analysis have been discussed to present new techniques in analyzing DNA contents and cell-cycle specific proteins.

Source: Chinese Journal of Analytical Chemistry - September 13, 2019 Category: Chemistry Source Type: research