Selenoprotein W Deficiency Does Not Affect Oxidative Stress and Insulin Sensitivity in the Skeletal Muscle of High-fat Diet-fed Obese Mice.

Selenoprotein W Deficiency Does Not Affect Oxidative Stress and Insulin Sensitivity in the Skeletal Muscle of High-fat Diet-fed Obese Mice. Am J Physiol Cell Physiol. 2019 Sep 11;: Authors: Shin MG, Cha HN, Park S, Kim YW, Kim JY, Park SY Abstract Selenoprotein W (SelW) is a selenium containing protein with a redox motif found abundantly in the skeletal muscle of rodents. Previous in vitro studies suggest that SelW plays an antioxidant role; however, relatively few in vivo studies have addressed the antioxidant role of SelW. Since oxidative stress is a causative factor for the development of insulin resistance in obese subjects, we hypothesized that if SelW plays a role as an antioxidant, SelW deficiency could aggravate the oxidative stress and insulin resistance caused by a high-fat diet. SelW deficiency did not affect insulin sensitivity and H2O2 levels in the skeletal muscle of control diet-fed mice. SelW levels in the skeletal muscle were decreased by high-fat diet feeding for 12 weeks. High-fat diet induced obesity and insulin resistance and increased the levels of H2O2 and oxidative stress makers, which were not affected by SelW deficiency. High-fat diet feeding increased the expression of antioxidant enzymes; however, SelW deficiency did not affect the expression levels of antioxidants. These results suggest that SelW does not play a protective role against oxidative stress and insulin resistance in the skeletal muscle of high...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research