Activities of Calcium-Related Ion Channels during the Formation of Kidney Stones in an Infection-Induced Urolithiasis Rat Model.

In this study, we hypothesized another possible method of bacteria contributing to the formation of calcium oxalate (CaOx) that accounts for the biggest part of the kidney stone. Bacteria may play important roles by influencing renal calcium-related ion channel activities, resulting in chronic inflammation of the kidney along with the rapid aggregation of stones. We examined the correlation among infection-promoted CaOx kidney stones and alterations in calcium-related ion channels in an animal model with experimentally induced Proteus mirabilis and foreign-body infection. After infecting the bladder for 7 days, the data demonstrated that the stones were presented and induced severe renal tubular breakage as well as altered levels of MCP-1, COX-2, OPN, and TRPV5 expression, reflecting responses of the kidney ion channels. MCP-1, OPN, and TRPV5 expression were significantly downregulated over time, indicating the chronic inflammation phase of the kidney and accelerated aggregation of CaOx crystals, respectively, while COX-2 exhibited no differences. These results indicated that bacterial infection is considerably correlated with an alteration in renal calcium-related ion channels and might support specific targeted calcium-related ion channel-based therapeutics for urolithiasis and related inflammatory renal damage. PMID: 31509008 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Renal Physiology - Category: Physiology Authors: Tags: Am J Physiol Renal Physiol Source Type: research